Recombinant Rabbit Anti-NEFH Antibody (clone R07-3I6)
CAT#: VS3-FY1024
This product is a recombinant rabbit antibody that recognizes NEFH. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R07-3I6 is specific for human, mouse, rat NEFH. The antigen is human neurofilament heavy chain polypeptide recombinant protein.
Gene Expression
Normal Tissue
Figure 1 High expression in cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7786/20899_B_7_5.jpg
Normal Tissue
Figure 2 Colon
Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7786/20899_A_7_3.jpg
Normal Tissue
Figure 3 Liver
Hepatocytes Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7786/20899_A_9_4.jpg
Normal Tissue
Figure 4 Prostate
Glandular cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7786/20899_A_1_5.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enriched (SiHa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100285-NEFH
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Specifications
- Immunogen
- Human Neurofilament Heavy Chain Polypeptide Recombinant Protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat NEFH
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R07-3I6
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 112 kDa; Observed MW: 180-220 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "NEFH"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0719MZ | Chicken Anti-NEFH Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| BRD-1121MZ | Chicken Anti-Neurofilament H (NF-H) Polyclonal IgY | WB, IF | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1078-CN | Recombinant Rabbit Anti-NEFH Monoclonal Antibody (JF99-07) | WB, IHC | Rabbit IgG |
| ZG-1091F | Mouse Anti-NEFH Recombinant Antibody (clone RNF404) | IHC-Fr, IHC-P, WB | Mouse IgG2a |
| VS-0223-WK51 | Rabbit Anti-NEFH Recombinant Antibody (VS-0223-WK51) | ICC, IHC, IHC-P | Rabbit IgG |
| VS3-FY1022 | Recombinant Mouse Anti-NEFH Antibody (clone 2C12F3) | ELISA, IHC | Mouse IgG1 |
| VS3-FY1023 | Recombinant Mouse Anti-NEFH Antibody (clone 4F2G12) | ELISA, IHC, FC | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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