Recombinant Rabbit Anti-NLRP3 Antibody (clone R07-9E9)
CAT#: VS3-FY1046
This product is a recombinant rabbit antibody that recognizes NLRP3. This antibody has been reported for use in Western Blot. The clone R07-9E9 is specific for human NLRP3. The antigen is human nlrp3 recombinant protein.
Gene Expression
Tested Data
Normal Tissue
Figure 1 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in germinal and non-germinal center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12878/29164_A_6_8_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12878/29164_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12878/29164_A_8_3.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12878/29164_A_6_6.jpg
Normal Tissue
Figure 5 Bone marrow
Hematopoietic cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12878/29164_B_5_4.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12878/29164_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enriched (THP-1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162711-NLRP3
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WB
Figure 1. Recombinant Rabbit Anti-NLRP3 Antibody (clone R07-9E9) in WB.
Western Blot analysis of NLRP3 in THP-1 lysates using NLRP3 Antibody.
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Specifications
- Immunogen
- Human NLRP3 recombinant protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NLRP3
- Species Reactivity
- Human
- Clone
- R07-9E9
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 118 kDa; Observed MW: 118 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- NLR Family Pyrin Domain Containing 3; Cryopyrin; Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3; Cold-Induced Autoinflammatory Syndrome 1 Protein; PYRIN-Containing APAF1-Like Protein 1; Caterpiller Protein 1.1; C1orf7; CLR1.1; PYPAF1; CIAS1; NALP3; NACHT Domain-, Leucine-Rich Repeat-, And PYD-Containing Protein 3; NACHT, LRR And PYD Domains-Containing Protein 3
- Gene ID
- 114548
- UniProt ID
- Q96P20
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "NLRP3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2811z | Mouse Anti-NLRP3 Recombinant Antibody (MOB-2811z) | WB, IHC, IHC-Fr, IHC-P, IP | Mouse IgG1 |
| MOB-2139MZ | Recombinant Mouse Anti-Human NLRP3 Antibody (clone Obmqz4-b) | ICC, WB | Mouse antibody |
| MRO-1097-CN | Recombinant Rabbit Anti-NLRP3 Monoclonal Antibody (CBACN-403) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MRO-2076-CN | Rabbit Anti-NLRP3 Polyclonal Antibody (MRO-2076-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS13-YC816 | Rabbit Anti-NLRP3 Polyclonal Antibody (VS13-YC816) | WB, IHC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2811z-F(E) | Recombinant Anti-Human NLRP3 Antibody Fab Fragment | WB, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2811z-S(P) | Recombinant Anti-Human NLRP3 Antibody scFv Fragment | ELISA, IF, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2453 | Hi-Affi™ Recombinant Rabbit Anti-NLRP3 Monoclonal Antibody (DS2453AB) | FC, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1499 | Anti-NLRP3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4870 | Anti-Mouse NLRP3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4869 | Anti-Human NLRP3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC817 | CytoStream™ Rabbit Anti-NLRP3 Recombinant Antibody (VS13-YC817) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
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