Recombinant Mouse Anti-PERK Antibody (clone 4A3A10)
CAT#: VS4-CJ72
This mouse anti-PERK antibody (clone 4A3A10) is a product that recognizes human PERK. The immunogen of this antibody is recombinant human Eukaryotic translation initiation factor 2-alpha kinase 3 protein (115aa-330aa). This product was purified by protein G purified and it has been tested for use in ELISA, Immunofluorescence (1:50-1:200).
Specifications
- Immunogen
- Recombinant Human Eukaryotic translation initiation factor 2-alpha kinase 3 protein (115aa-330aa)
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PERK
- Species Reactivity
- Human
- Clone
- 4A3A10
- Applications
- ELISA, IF
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.01M PBS, pH 7.4.
- Preservative
- 0.03% Proclin 300
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Immunofluorescence (1:50-1:200).
Target
- Alternative Names
- PEK; WRS; PERK
- Gene ID
- 9451
- UniProt ID
- Q9NZJ5
- Long Name
- Eukaryotic translation initiation factor 2 alpha kinase 3
- Sequence Similarities
- Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
- Cellular Localization
- Endoplasmic reticulum, Membrane
- Post Translation Modifications
- Oligomerization of the N-terminal ER luminal domain by ER stress promotes PERK trans-autophosphorylation of the C-terminal cytoplasmic kinase domain at multiple residues including Thr-982 on the kinase activation loop (By similarity).
Autophosphorylated. Phosphorylated at Tyr-619 following endoplasmic reticulum stress, leading to activate its tyrosine-protein kinase activity. Dephosphorylated by PTPN1/TP1B, leading to inactivate its enzyme activity.
N-glycosylated.
DP-ribosylated by PARP16 upon ER stress, which increases kinase activity.
- Protein Refseq
- NP_004827.4
- Function
- Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions. Key activator of the integrated stress response (ISR) required for adaptation to various stress, such as unfolded protein response (UPR) and low amino acid availability (By similarity).
EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming (PubMed:33384352).
Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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