Recombinant Rabbit Anti-PFN1 Antibody (clone R06-6H7)
CAT#: VS3-FY1128
This product is a recombinant rabbit antibody that recognizes PFN1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R06-6H7 is specific for human, mouse, rat PFN1. The antigen is human profilin 1 synthetic peptide.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37140/if_selected.jpg
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WB
Figure 1. Recombinant Rabbit Anti-PFN1 Antibody (clone R06-6H7) in WB.
Western Blot analysis of Profilin 1 in C6, 3T3, Hela lysates using Profilin 1 Antibody.
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Specifications
- Immunogen
- Human Profilin 1 Synthetic Peptide.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat PFN1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-6H7
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 15 kDa; Observed MW: 15 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "PFN1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2203z | Mouse Anti-PFN1 Recombinant Antibody (clone 2B6) | WB, FC, ICC, IF, IHC | Mouse IgG2a |
| MRO-1273-CN | Recombinant Rabbit Anti-PFN1 Monoclonal Antibody (JA30-13) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-WK242 | Rabbit Anti-PFN1 Recombinant Antibody (clone JA30-13) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2673 | Hi-Affi™ Recombinant Rabbit Anti-PFN1 Monoclonal Antibody (DS2673AB) | WB, IHC, ICC, IP, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY411 | Mouse Anti-NHP PFN1 Recombinant Antibody (clone OTI2D2) | WB, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1626 | Anti-PFN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5329 | Anti-Mouse PFN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5328 | Anti-Human PFN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5330 | Anti-Canine PFN1 Immunohistochemistry Kit | IHC |
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