Recombinant Rabbit Anti-PHLDA1 Antibody (clone R02-9F4) (CAT#: VS3-FY1137)
This product is a recombinant rabbit antibody that recognizes PHLDA1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R02-9F4 is specific for human PHLDA1. The antigen is synthetic peptide of human phlda1.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & nucleoli rim.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63520/1185_C1_5_selected.jpg
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & nucleoli rim.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63520/1185_C1_5_selected.jpg
Figure 2 Cerebral cortex
(Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19000/43956_B_8_5.jpg
(Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19000/43956_B_8_5.jpg
Figure 3 Salivary gland
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19000/43956_B_2_1.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19000/43956_B_2_1.jpg
Figure 4 Colon
(Endocrine cells Staining: Medium Intensity: Moderate Enterocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Strong Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19000/43956_A_9_3.jpg
(Endocrine cells Staining: Medium Intensity: Moderate Enterocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Strong Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19000/43956_A_9_3.jpg
Figure 5 Liver
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19000/43956_A_9_4.jpg
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19000/43956_A_9_4.jpg
Figure 6 RNA cell line category: Cell line enhanced (hTERT-RPE1, RPTEC TERT1)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000139289-PHLDA1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000139289-PHLDA1
Specifications
- Immunogen
- Synthetic peptide of human PHLDA1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PHLDA1
- Species Reactivity
- Human
- Clone
- R02-9F4
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 45 kDa; Observed MW: 45 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Pleckstrin Homology Like Domain Family A Member 1; T-Cell Death-Associated Gene 51 Protein; Apoptosis-Associated Nuclear Protein; Proline- And Glutamine-Rich Protein; Proline- And Histidine-Rich Protein; Proline-Histidine Rich Protein; PQ-Rich Protein
- Gene ID
- 22822
- UniProt ID
- Q8WV24
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2694 | Hi-Affi™ Recombinant Rabbit Anti-PHLDA1 Monoclonal Antibody (DS2694AB) | WB, IHC, ICC | IgG |
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For Research Use Only. Not For Clinical Use.
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