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Recombinant Rabbit Anti-PIN1 Antibody (clone R01-7G8)

Recombinant Rabbit Anti-PIN1 Antibody (clone R01-7G8) (CAT#: VS3-FY1144)

Name: Recombinant Rabbit Anti-PIN1 Antibody (clone R01-7G8)
SKU: VS3-FY1144
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes PIN1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R01-7G8 is specific for human, mouse, rat, Hamster PIN1. The antigen is pin1 recombinant protein.

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1. Recombinant Rabbit Anti-PIN1 Antibody (clone R01-7G8) in WB.

Figure 1. Recombinant Rabbit Anti-PIN1 Antibody (clone R01-7G8) in WB.

Western Blot analysis of Pin1 in Jurkat, Rat Brain, C6, CHO-K1, Hela lysates using Pin1 Antibody.

IHC-P

Figure 2. Recombinant Rabbit Anti-PIN1 Antibody (clone R01-7G8) in IHC-P.

Figure 2. Recombinant Rabbit Anti-PIN1 Antibody (clone R01-7G8) in IHC-P.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Pin1 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.

Subcellular Location

Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68650/1578_E7_3_selected.jpg

Figure 2 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70887/1578_G7_1_selected.jpg

Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4528/if_selected.jpg

Figure 4 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9326/if_selected.jpg

Normal Tissue

Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4528/16262_A_6_6.jpg

Figure 6 Cerebral cortex
(Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4528/16262_B_7_5.jpg

Figure 7 Colon
(Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4528/16262_A_9_3.jpg

RNA Expression

Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000127445-PIN1

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Pin1 recombinant protein.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Mouse, Rat, Hamster PIN1

Species Reactivity
Human, Mouse, Rat, Hamster

Clone
R01-7G8

Applications
Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Conjugate
Unconjugated

MW
Calculated MW: 18 kDa; Observed MW: 18 kDa

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20

Target

Alternative Names
Peptidylprolyl Cis/Trans Isomerase, NIMA-Interacting 1; Protein (Peptidyl-Prolyl Cis/Trans Isomerase) NIMA-Interacting 1; Rotamase Pin1; PPIase Pin1; EC 5.2.1.8; Peptidyl-Prolyl Cis-Trans Isomerase NIMA-Interacting 1; Peptidyl-Prolyl Cis-Trans Isomerase Pin1; UBL5; DOD

Gene ID
5300

UniProt ID
Q13526

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "PIN1"

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Please select product type

IgG Antibody Products Chicken IgY Antibody Products Rabbit Monoclonal Antibody Products Non-Human Primate (NHP) Antibody Products

CAT	Product Name	Application	Type
MOB-2511z	Mouse Anti-PIN1 Recombinant Antibody (clone 37A10)	WB, ICC, IHC, IP	Mouse IgG2b
MOB-1600CT	Recombinant Mouse anti-Human PIN1 Monoclonal antibody (4H9)	ELISA, WB
VS3-QX878	Mouse Anti-PIN1 Recombinant Antibody (clone 855CT1.7.5)	WB, IHC, FC	Mouse IgG1
VS3-WK940	Mouse Anti-PIN1 Recombinant Antibody (VS3-WK940)	FC, WB, IHC, IHC-P	Mouse IgG

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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