Recombinant Rabbit Anti-PRDX1 Antibody (clone R04-0H5)
CAT#: VS3-FY1179
This product is a recombinant rabbit antibody that recognizes PRDX1. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R04-0H5 is specific for human, mouse PRDX1. The antigen is synthetic peptide of human peroxidase 1/pag.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7730/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human thyroid gland
Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7730/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7730/11_C9_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7730/15_C9_1_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Glial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4682/11479_B_7_5.jpg
Normal Tissue
Figure 6 Thyroid gland
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4682/11479_B_2_5.jpg
Normal Tissue
Figure 7 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4682/11479_A_9_3.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4682/11479_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4682/11479_A_5_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4682/11479_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000117450-PRDX1
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ICC
Figure 1. Recombinant Rabbit Anti-PRDX1 Antibody (clone R04-0H5) in ICC.
Immunocytochemical analysis of peroxidase 1 (green) in Hela using peroxidase 1 antibody and DAPI (blue).
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Specifications
- Immunogen
- Synthetic peptide of human peroxidase 1/PAG.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse PRDX1
- Species Reactivity
- Human, Mouse
- Clone
- R04-0H5
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 22 kDa; Observed MW: 22 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "PRDX1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1986-sdAb | Recombinant Anti-Human PRDX1 VHH Single Domain Antibody | IP, ChiP, IHC, ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0984MZ | Recombinant Mouse Anti-Human PRDX1 Antibody | WB | Mouse antibody |
| MRO-1206-CN | Recombinant Rabbit Anti-PRDX1 Monoclonal Antibody (JF0945) | WB, IF, IHC, FC | Rabbit IgG |
| MOB-0188F | Mouse Anti-PRDX1 Recombinant Antibody (clone 8E7) | WB, IHC-P, IF | Mouse IgG |
| VS3-QX911 | Mouse Anti-PRDX1 Recombinant Antibody (clone 6A6-4H3-7A3) | WB, IF | Mouse IgG1 |
| VS3-WK890 | Mouse Anti-PRDX1 Recombinant Antibody (VS3-WK890) | WB, IF, ICC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2836 | Hi-Affi™ Recombinant Rabbit Anti-PRDX1 Monoclonal Antibody (DS2836AB) | FC, ICC, IF, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1268 | AbPlus™ Anti-PRDX1 Magnetic Beads (VS-0724-YC1268) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5653 | Anti-PRDX1 Immunohistochemistry Kit | IHC |
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