Recombinant Rabbit Anti-PRKCA Antibody (clone R05-4E5) (CAT#: VS3-FY1189)
This product is a recombinant rabbit antibody that recognizes PRKCA. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R05-4E5 is specific for human, mouse, rat PRKCA. The antigen is synthetic peptide of human pkcα.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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WB
Figure 1. Recombinant Rabbit Anti-PRKCA Antibody (clone R05-4E5) in WB.
Western Blot analysis of PKC α in K562, C6, 3T3, Hela lysates using PKC α Antibody.
IHC-P
Figure 2. Recombinant Rabbit Anti-PRKCA Antibody (clone R05-4E5) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using PKC α antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
Specifications
- Immunogen
- Synthetic peptide of human PKCα.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat PRKCA
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R05-4E5
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 77 kDa; Observed MW: 80 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- PRKCA; PKCA; PRKACA; Protein kinase C alpha type; PKC-A; PKC-alpha
- Gene ID
- 5578
- UniProt ID
- P17252
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane, Mitochondrion, Nucleus
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1687z | Mouse Anti-PRKCA Recombinant Antibody (clone 20A4) | WB, ELISA | Mouse IgG2a |
| MRO-1235-CN | Recombinant Rabbit Anti-PRKCA Monoclonal Antibody (CBACN-450) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-0333U | Rabbit Anti-PRKCA Recombinant Antibody (clone G13-R) | ELISA, WB, IP, ICC | Rabbit IgG |
| VS3-CJ90 | Mouse Anti-PRKCA Recombinant Antibody (VS3-CJ90) | WB, ELISA | Mouse IgG |
| VS3-WK2037 | Rabbit Anti-PRKCA (pS657) Recombinant Antibody (clone R05-8I3) | WB, IHC-P | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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