Recombinant Anti-PROM1 Vesicular Antibody, EV Displayed (VS-0425-YC266)

Name: Recombinant Anti-PROM1 Vesicular Antibody, EV Displayed (VS-0425-YC266)
SKU: VS-0425-YC266
Rating: 5 (1 reviews)

CAT#: VS-0425-YC266

The Recombinant Anti-PROM1 Vesicular Antibody, EV Displayed (VS-0425-YC266) is an antibody-displaying extracellular vesicle (Ab-EV). The product combines the benefits of both extracellular vesicle (EV) and antibody (Ab) which can guide the decorated EVs to PROM1-expressed cells or tissues. The PROM1 is a glycoprotein maintaining stem cell properties and associated with retinal diseases and cancer.

Gene Expression

Normal Tissue Figure 1 Colon

Goblet cells
Staining:Medium
Intensity: Moderate
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4922/15212_A_8_3.jpg

Normal Tissue Figure 2 Liver

Cholangiocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4922/15212_A_7_4.jpg

Normal Tissue Figure 3 Pancreas

Exocrine glandular cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4922/15212_A_2_3.jpg

Normal Tissue Figure 4 Kidney

Bowman's capsule
Staining:High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4922/15212_A_8_5.jpg

RNA Expression Figure 5 RNA cell line category: Cell line enhanced (AF22, CACO-2, CAPAN-2, EFO-21, NTERA-2, RPTEC TERT1)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000007062-PROM1

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Recombinant Antibody

Application
ELISA, FC, Cell-uptake

Product Type
Ab-Fc-EVs

Antibody Quantification (Ab/EV)
~100 Ab/EV

Target
PROM1

Host Animal
Mouse

Antibody Isotype
IgG

Species Reactivity
Human

Expression Cell
Mammalian cell

Engineered EVs

EV-sorting domain
CD63

Fc-binding domain
Protein A

EV Size
30~150 nm

Producing Cell
HEK293F

Isolation Method
Gradient centrifugation

Purification
qEV size exclusion chromatography

Concentration
1 x 10¹⁰

Size
1 mL

Buffer
PBS

Storage
Store at -80°C for 12 months

Target

Full Name
Prominin 1

Cellular Localization
Plasma membrane

Introduction
This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner.

Alternative Names
AC133, CD133, CORD12, MCDR2, PROML1, RP41, STGD4

Gene ID
8842

UniProt ID
O43490

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Datasheet

Datasheet - VS-0425-YC266.pdf

MSDS

MSDS - VS-0425-YC266.pdf

COA

Certificate of Analysis Lookup

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