Recombinant Rabbit Anti-PXN Antibody (clone R04-3C3) (CAT#: VS3-FY1230)
This product is a recombinant rabbit antibody that recognizes PXN. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R04-3C3 is specific for human, mouse, rat PXN. The antigen is synthetic peptide of human paxillin.
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WB
Figure 1. Recombinant Rabbit Anti-PXN Antibody (clone R04-3C3) in WB.
Western Blot analysis of paxillin in C6 lysates using paxillin antibody.
IHC-P
Figure 2. Recombinant Rabbit Anti-PXN Antibody (clone R04-3C3) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Paxillin Antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
Specifications
- Immunogen
- synthetic peptide of human paxillin.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat PXN
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-3C3
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 65 kDa; Observed MW: 65 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- PXN; Paxillin
- Gene ID
- 5829
- UniProt ID
- P49023
- Sequence Similarities
- Belongs to the paxillin family.
- Cellular Localization
- Cell junction, Cytoplasm, Cytoskeleton
- Post Translation Modifications
- Phosphorylated by MAPK1/ERK2 (By similarity).
Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. Phosphorylation at Ser-250 by SLK is required for PXN redistribution and cell motility (PubMed:23128389).
- Protein Refseq
- NP_001074324.1; NP_001230685.1; NP_002850.2
- Function
- Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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ScFv Antibody Products
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Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-314 | Human Anti-PXN Recombinant Antibody (clone CBL14Q); scFv Fragment | WB, ELISA, IF, FuncS | Human scFv |
| PSBL-668 | Human Anti-PXN Recombinant Antibody (clone CBL19Q); scFv Fragment | WB, FuncS | Human scFv |
| PSBL-669 | Human Anti-PXN Recombinant Antibody (clone CBL35Q); scFv Fragment | WB, FuncS | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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