Recombinant Rabbit Anti-RAB5C Antibody (clone R04-3B7)
CAT#: VS3-FY1234
This product is a recombinant rabbit antibody that recognizes RAB5C. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The antigen is human RAB5C recombinant protein. The clone R04-3B7 is specific for human, mouse, rat RAB5C.
Tested Data
WB
Figure 1. Recombinant Rabbit Anti-RAB5C Antibody (clone R04-3B7) in WB.
Western Blot analysis of RAB5C in K562, C6, 3T3, Hela lysates using RAB5C Antibody.
IHC-P
Figure 2. Recombinant Rabbit Anti-RAB5C Antibody (clone R04-3B7) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human cholangiocarcinoma using RAB5C antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Human RAB5C recombinant protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat RAB5C
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-3B7
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 23 kDa; Observed MW: 23 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- RAB5C, Member RAS Oncogene Family; RAB5C, Member Of RAS Oncogene Family; L1880; RAB5L; RABL; RAB, Member Of RAS Oncogene Family-Like; Ras-Related Protein Rab-5C; RAB5CL
- Gene ID
- 5878
- UniProt ID
- P51148
- Sequence Similarities
- Belongs to the small GTPase superfamily. Rab family.
- Cellular Localization
- Cell membrane, Endosome, Membrane
- Post Translation Modifications
- Phosphorylation of Ser-85 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2.
(Microbial infection) Glycosylated on arginine residues by S.typhimurium protein Ssek3.
- Protein Refseq
- NP_001238968.1; NP_004574.2; NP_958842.1
- Function
- Protein transport. Probably involved in vesicular traffic.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "RAB5C"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ1012 | Mouse Anti-RAB5C Recombinant Antibody (VS3-CJ1012) | WB, FC | Mouse IgG2b, κ |
| VS13-YC970 | Rabbit Anti-RAB5C Recombinant Antibody (VS13-YC970) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5932 | Anti-RAB5C Immunohistochemistry Kit | IHC |
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