Recombinant Rabbit Anti-RAB5C Antibody (clone R04-3B7) (CAT#: VS3-FY1234)

This product is a recombinant rabbit antibody that recognizes RAB5C. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The antigen is human RAB5C recombinant protein. The clone R04-3B7 is specific for human, mouse, rat RAB5C.


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WB

Figure 1. Recombinant Rabbit Anti-RAB5C Antibody (clone R04-3B7) in WB.

Figure 1. Recombinant Rabbit Anti-RAB5C Antibody (clone R04-3B7) in WB.

Western Blot analysis of RAB5C in K562, C6, 3T3, Hela lysates using RAB5C Antibody.

IHC-P

Figure 2. Recombinant Rabbit Anti-RAB5C Antibody (clone R04-3B7) in IHC-P.

Figure 2. Recombinant Rabbit Anti-RAB5C Antibody (clone R04-3B7) in IHC-P.

Immunohistochemical analysis of paraffin-embedded human cholangiocarcinoma using RAB5C antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.


Specifications

  • Immunogen
  • Human RAB5C recombinant protein.
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse, Rat RAB5C
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R04-3B7
  • Applications
  • Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
  • Conjugate
  • Unconjugated
  • MW
  • Calculated MW: 23 kDa; Observed MW: 23 kDa

Product Property

  • Purification
  • Affinity Purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry-Paraffin: 1/50-1/100
    Immunofluorescence: 1/50-1/200

Target

  • Alternative Names
  • RAB5C, Member RAS Oncogene Family; RAB5C, Member Of RAS Oncogene Family; L1880; RAB5L; RABL; RAB, Member Of RAS Oncogene Family-Like; Ras-Related Protein Rab-5C; RAB5CL
  • Sequence Similarities
  • Belongs to the small GTPase superfamily. Rab family.
  • Cellular Localization
  • Cell membrane, Endosome, Membrane
  • Post Translation Modifications
  • Phosphorylation of Ser-85 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2.
    (Microbial infection) Glycosylated on arginine residues by S.typhimurium protein Ssek3.
  • Function
  • Protein transport. Probably involved in vesicular traffic.

Recommended Products

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "RAB5C"

Recombinant Antibody

CAT Product Name Application Type
VS3-CJ1012 Mouse Anti-RAB5C Recombinant Antibody (VS3-CJ1012) WB, FC Mouse IgG2b, κ

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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