Recombinant Rabbit Anti-RACK1 Antibody (clone R02-1K6)
CAT#: VS3-FY1239
This product is a recombinant rabbit antibody that recognizes RACK1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin. The clone R02-1K6 is specific for human, mouse, rat RACK1. The antigen is synthetic peptide of human rack1.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21676/299_D4_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human breast
Immunohistochemical staining of human breast shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4288/51894_B_2_4_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4288/51894_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4288/51894_A_7_3.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4288/51894_A_4_6.jpg
Normal Tissue
Figure 6 Fallopian tube
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4288/51894_A_8_7.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4288/51894_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204628-RACK1
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WB
Figure 1. Recombinant Rabbit Anti-RACK1 Antibody (clone R02-1K6) in WB.
Western Blot analysis of RACK1 in C6, 3T3, Hela lysates using RACK1 Antibody.
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Specifications
- Immunogen
- Synthetic peptide of human RACK1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat RACK1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-1K6
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- Calculated MW: 35 kDa; Observed MW: 35 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Target
- Alternative Names
- Receptor For Activated C Kinase 1; Guanine Nucleotide Binding Protein (G Protein), Beta Polypeptide 2-Like 1; Guanine Nucleotide-Binding Protein Subunit Beta-Like Protein 12.3; Guanine Nucleotide-Binding Protein Subunit Beta-2-Like 1; Cell Proliferation-Inducing Gene 21 Protein; Small Ribosomal Subunit Protein RACK1; Human Lung Cancer Oncogene 7 Protein; GNB2L1; HLC-7; Protein Homologous To Chicken B Complex Protein, Guanine Nucleotide Binding
- Gene ID
- 10399
- UniProt ID
- P63244
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "RACK1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1466 | Hi-Affi™ Recombinant Rabbit Anti-GNB2L1 Monoclonal Antibody (DS1466AB) | WB, IHC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC3043 | PE-Gogo-B*0101/Human RACK1 (SRDKTIIMW) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1312-CN | Recombinant Rabbit Anti-RACK1 Monoclonal Antibody (JG40-26) | WB, IHC, FC | Rabbit IgG |
| VS3-WK503 | Rabbit Anti-RACK1 Recombinant Antibody (clone JG40-26) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1860 | Anti-RACK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5948 | Anti-Mouse RACK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5947 | Anti-Human RACK1 Immunohistochemistry Kit | IHC |
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