Recombinant Rabbit Anti-RAP1GAP Antibody (clone R08-9F6)
CAT#: VS3-FY1248
This product is a recombinant rabbit antibody that recognizes RAP1GAP. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R08-9F6 is specific for human, mouse, rat RAP1GAP. The antigen is synthetic peptide of human rap1gap.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3851/if_selected.jpg
Normal Tissue
Figure 2 High expression in caudate nucleus
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1922/167870_B_8_7_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1922/167870_B_9_5.jpg
Normal Tissue
Figure 4 Caudate
Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1922/167870_B_9_7.jpg
Normal Tissue
Figure 5 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1922/167870_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1922/167870_A_8_5.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1922/167870_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (A549, OE19, SCLC-21H)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000076864-RAP1GAP
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Specifications
- Immunogen
- Synthetic peptide of human RAP1GAP.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat RAP1GAP
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R08-9F6
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 73 kDa; Observed MW: 95 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "RAP1GAP"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2646z | Mouse Anti-RAP1GAP Recombinant Antibody (clone 40G6) | WB, ELISA, IF, IHC, IP | Mouse IgG2a, κ |
| MOB-1340CT | Recombinant Mouse anti-Human RAP1GAP Monoclonal antibody (EML2023) | IHC-P, WB | |
| VS3-XY1357 | Mouse Anti-RAP1GAP Recombinant Antibody (clone 2G7B6) | ELISA, WB, FC | Mouse IgG2b |
| VS3-XY1358 | Mouse Anti-RAP1GAP Recombinant Antibody (clone 9A12D8) | ELISA, WB, FC | Mouse IgG2b |
| VS3-FY1247 | Recombinant Rabbit Anti-RAP1GAP Antibody (clone R06-9H2) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2999 | Hi-Affi™ Recombinant Rabbit Anti-RAP1GAP Monoclonal Antibody (DS2999AB) | WB, FC, IP, IHC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5983 | Anti-RAP1GAP Immunohistochemistry Kit | IHC |
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