Recombinant Rabbit Anti-S100A6 Antibody (clone R01-8A4)
CAT#: VS3-FY1298
This product is a recombinant rabbit antibody that recognizes S100A6. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R01-8A4 is specific for human, mouse S100A6. The antigen is human s100α6/pra recombinant protein.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7575/18_B10_1_selected.jpg
Normal Tissue
Figure 2 Urinary bladder
High expression in urinary bladder
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7575/21633_A_4_5_val_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40549/84815_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2601/6726_A_9_4.jpg
Normal Tissue
Figure 5 Pancreas
Exocrine glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2601/6726_A_3_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2601/6726_A_8_5.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2601/6726_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (OE19, RPTEC TERT1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000197956-S100A6
❮
❯
ICC
Figure 1. Recombinant Rabbit Anti-S100A6 Antibody (clone R01-8A4) in ICC.
Immunocytochemical analysis of S100 alpha 6/PRA (green) in hela using S100 alpha 6/PRA Antibody and DAPI (blue).
❮
❯
Specifications
- Immunogen
- Human S100α6/PRA recombinant protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse S100A6
- Species Reactivity
- Human, Mouse
- Clone
- R01-8A4
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 10 kDa; Observed MW: 10 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- S100 Calcium Binding Protein A6; Prolactin Receptor-Associated Protein; Growth Factor-Inducible Protein 2A9; Calcyclin; MLN 4; CACY; PRA; S100 Calcium-Binding Protein A6 (Calcyclin)
- Gene ID
- 6277
- UniProt ID
- P06703
- Sequence Similarities
- Belongs to the S-100 family.
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane, Nucleus
- Post Translation Modifications
- The N-terminus is blocked.
- Protein Refseq
- NP_055439.1
- Function
- May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY1298. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "S100A6"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1198CT | Recombinant Mouse anti-Human S100A6 Monoclonal antibody (EML679) | IHC-P, WB | |
| VS3-QX995 | Mouse Anti-S100A6 Recombinant Antibody (clone 3E11-E9-D8) | WB | Mouse IgG1 |
| VS3-WK407 | Rabbit Anti-S100A6 Recombinant Antibody (clone JF0976) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS3-CJ441 | Mouse Anti-S100A6 Recombinant Antibody (clone PT2233) | IHC-P | Mouse IgG1, κ |
| VS3-CJ442 | Mouse Anti-S100A6 Recombinant Antibody (clone ABT-S100A6) | IHC-P | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0523MZ | Chicken Anti-S100A6 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3156 | Hi-Affi™ Recombinant Rabbit Anti-S100A6 Monoclonal Antibody (DS3156AB) | ELISA, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC490 | AbPlus™ Anti-S100A6 Magnetic Beads (VS-0724-YC490) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1993 | Anti-S100A6 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6306 | Anti-Mouse S100A6 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6307 | Anti-Rat S100A6 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, WB
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: WB, IHC, FC, Cyt, ELISA
Application: Neut, Inhib, FC, ELISA
Application: WB, FC, IF, Inhib, ELISA, IHC
Application: Neut, IHC, Activ, FuncS, IF, ELISA
Application: ELISA, FC, Neut, Inhib
Application: IF, ICC, WB, IHC-P, IP
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
