Recombinant Rabbit Anti-S100A9 Detection Antibody (clone R31-4D-5)
CAT#: VS3-WK1927
This antibody is a rabbit recombinant antibody against human S100A9. It was developed by immunization of rabbit with recombinant human S100A9 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. R19-5C-9 and R31-4D-5 were identified as antibody pair for sandwich ELISA. As a detection antibody, the clone R31-4D-5 can be used in ELISA analysis to identify human S100A9.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/33_B12_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_B_8_5.jpg
Normal Tissue
Figure 3 Esophagus
Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_B_6_1.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_A_9_3.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enriched (SK-BR-3)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000163220-S100A9
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Specifications
- Immunogen
- Recombinant human S100A9 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human S100A9
- Species Reactivity
- Human
- Clone
- R31-4D-5
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- S100 Calcium Binding Protein A9; Migration Inhibitory Factor-Related Protein 14; Leukocyte L1 Complex Heavy Chain; Calprotectin L1H Subunit; MRP-14; MRP14; CFAG; CAGB; P14; S100 Calcium-Binding Protein A9 (Calgranulin B); S100 Calcium Binding Protein A9 (Calgranulin B); S100 Calcium-Binding Protein A9
- Gene ID
- 6280
- UniProt ID
- P06702
- Long Name
- S100 Calcium Binding Protein A9
- Sequence Similarities
- Belongs to the S-100 family.
- Cellular Localization
- Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Secreted
- Post Translation Modifications
- Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
S-nitrosylation of Cys-3 is implicated in LDL(ox)-induced S-nitrosylation of GAPDH at 'Cys-247' through a transnitrosylase mechanism involving a iNOS-S100A8/9 complex.
Methylation at His-105 by METTL9 reduces zinc-binding without affecting heterodimerization with S100A8.
- Protein Refseq
- NP_002956.1
- Function
- S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Capture antibody
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "S100A9"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3159 | Hi-Affi™ Recombinant Rabbit Anti-S100A9 Monoclonal Antibody (DS3159AB) | ICC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0323-CN | Mouse Anti-S100A9 Recombinant Antibody (clone IID4B10) | WB, ELISA, IHC | Mouse IgG1, κ |
| MRO-1359-CN | Recombinant Rabbit Anti-S100A9 Monoclonal Antibody (JF096-8) | WB, IF, IHC | Rabbit IgG |
| HPAB-2539LY | Mouse Anti-S100A9 Recombinant Antibody (HPAB-2539LY) | ELISA, Block, WB | Mouse IgG |
| MOB-0810-YJ | Mouse Anti-Mouse S100A9 Recombinant Antibody (CBS104D) | IHC-Fr, FuncS, IA, IF, IHC-P, WB | Mouse IgG1 |
| ZG-0556U | Rabbit Anti-S100A9 Recombinant Antibody (clone 2E9) | ELISA, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0323-CN-S(P) | Mouse Anti-S100A9 Recombinant Antibody (clone IID4B10); scFv Fragment | WB, ELISA, IHC | Mouse scFv |
| HPAB-2539LY-S(P) | Mouse Anti-S100A9 Recombinant Antibody; scFv Fragment (HPAB-2539LY-S(P)) | ELISA, Block | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0323-CN-F(E) | Mouse Anti-S100A9 Recombinant Antibody (clone IID4B10); Fab Fragment | WB, ELISA, IHC | Mouse Fab |
| HPAB-2539LY-F(E) | Mouse Anti-S100A9 Recombinant Antibody; Fab Fragment (HPAB-2539LY-F(E)) | ELISA, Block | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1995 | Anti-S100A9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6317 | Anti-Mouse S100A9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6318 | Anti-Rat S100A9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6316 | Anti-Human S100A9 Immunohistochemistry Kit | IHC |
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