Recombinant Rabbit Anti-S100A9 Detection Antibody (clone R31-4D-5) (CAT#: VS3-WK1927)
This antibody is a rabbit recombinant antibody against human S100A9. It was developed by immunization of rabbit with recombinant human S100A9 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. R19-5C-9 and R31-4D-5 were identified as antibody pair for sandwich ELISA. As a detection antibody, the clone R31-4D-5 can be used in ELISA analysis to identify human S100A9.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/33_B12_2_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/33_B12_2_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_B_8_5.jpg
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_B_8_5.jpg
Figure 3 Esophagus
(Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_B_6_1.jpg
(Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_B_6_1.jpg
Figure 4 Colon
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_A_9_3.jpg
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_A_9_3.jpg
Figure 5 RNA cell line category: Cell line enriched (SK-BR-3)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000163220-S100A9
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000163220-S100A9
Specifications
- Immunogen
- Recombinant human S100A9 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human S100A9
- Species Reactivity
- Human
- Clone
- R31-4D-5
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- S100 Calcium Binding Protein A9; Migration Inhibitory Factor-Related Protein 14; Leukocyte L1 Complex Heavy Chain; Calprotectin L1H Subunit; MRP-14; MRP14; CFAG; CAGB; P14; S100 Calcium-Binding Protein A9 (Calgranulin B); S100 Calcium Binding Protein A9 (Calgranulin B); S100 Calcium-Binding Protein A9
- Gene ID
- 6280
- UniProt ID
- P06702
- Long Name
- S100 Calcium Binding Protein A9
- Sequence Similarities
- Belongs to the S-100 family.
- Cellular Localization
- Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Secreted
- Post Translation Modifications
- Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
S-nitrosylation of Cys-3 is implicated in LDL(ox)-induced S-nitrosylation of GAPDH at 'Cys-247' through a transnitrosylase mechanism involving a iNOS-S100A8/9 complex.
Methylation at His-105 by METTL9 reduces zinc-binding without affecting heterodimerization with S100A8.
- Protein Refseq
- NP_002956.1
- Function
- S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1452MZ | Recombinant Mouse Anti-Human S100A9 Antibody (clone OP.245) | ELISA, sELISA | Mouse antibody |
| ZG-0556U | Rabbit Anti-S100A9 Recombinant Antibody (clone 2E9) | ELISA, IHC | Rabbit IgG |
| VS3-WK1926 | Recombinant Rabbit Anti-S100A9 Capture Antibody (clone R19-5C-9) | ELISA | Rabbit IgG |
| VS13-YC1030 | Rabbit Anti-S100A9 Recombinant Antibody (VS13-YC1030) | WB, IHC-P, ICC, IF | Rabbit IgG |
| VS7-0425-WR813 | Mouse Anti-S100A9 Recombinant Antibody (clone 3A1H2) | IHC, FC | Mouse IgG1 |
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