Recombinant Rabbit Anti-SIRT2 Antibody (clone R01-3F3)
CAT#: VS3-FY1339
This product is a recombinant rabbit antibody that recognizes SIRT2. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R01-3F3 is specific for human, mouse, rat SIRT2. The antigen is human sirt2 recombinant protein.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoli & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11165/89_D2_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4573/673_G5_2_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4573/151748_B_8_5.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4573/151748_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4573/151748_A_4_6.jpg
Normal Tissue
Figure 6 Skeletal muscle
Myocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4573/151748_B_2_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000068903-SIRT2
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WB
Figure 1. Recombinant Rabbit Anti-SIRT2 Antibody (clone R01-3F3) in WB.
Western Blot analysis of SIRT2 in rat brain lysates using SIRT2 antibody.
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Specifications
- Immunogen
- Human SIRT2 recombinant protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat SIRT2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-3F3
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 43 kDa; Observed MW: 39 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Sirtuin 2; Regulatory Protein SIR2 Homolog 2; SIR2-Like Protein 2; SIR2L2; SIR2L; Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 2 (S. Cerevisiae); Sirtuin (Silent Mating Type Information Regulation 2, S.Cerevisiae, Homolog) 2; NAD-Dependent Protein Deacetylase Sirtuin-2
- Gene ID
- 22933
- UniProt ID
- Q8IXJ6
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "SIRT2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3268 | Hi-Affi™ Recombinant Rabbit Anti-SIRT2 Monoclonal Antibody (DS3268AB) | FC, ICC, IF, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1395-CN | Recombinant Rabbit Anti-SIRT2 Monoclonal Antibody (CBACN-505) | IF, IP, FC | Rabbit IgG |
| MRO-1396-CN | Recombinant Rabbit Anti-SIRT2 Monoclonal Antibody (CBACN-506) | WB | Rabbit IgG |
| ZG-088R | Mouse Anti-SIRT2 Recombinant Antibody (ZG-088R) | WB | Mouse IgG |
| VS3-QX1012 | Mouse Anti-SIRT2 Recombinant Antibody (clone 1D4-H11-H11) | WB, IF | Mouse IgG2b |
| VS3-WK1054 | Rabbit Anti-SIRT2 Recombinant Antibody (VS3-WK1054) | WB, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1056 | CytoStream™ Rabbit Anti-SIRT2 Recombinant Antibody (VS13-YC1056) | WB, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2071 | Anti-SIRT2 Immunohistochemistry Kit | IHC |
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