Recombinant Rabbit Anti-TFAP2C Antibody (clone R03-8I2)
CAT#: VS3-FY1438
This product is a recombinant rabbit antibody that recognizes TFAP2C. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R03-8I2 is specific for human TFAP2C. The antigen is human ap2γ recombinant protein.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/57076/968_C2_2_selected.jpg
Normal Tissue
Figure 2 Liver
Hepatocytes
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55179/130417_A_8_4.jpg
Normal Tissue
Figure 3 Skin
Hair follicles
Staining: High
Intensity: Strong
Quantity: 75%-25%
Langerhans cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55179/130417_B_7_1.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (BEWO, MCF7, SK-BR-3, T-47d, WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000087510-TFAP2C/subcellular
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WB
Figure 1. Recombinant Rabbit Anti-TFAP2C Antibody (clone R03-8I2) in WB.
Western Blot analysis of TFAP2C/AP2 Gamma in Jurkat, Hela lysates using Transcription Factor AP 2 Gamma Antibody.
ICC
Figure 2. Recombinant Rabbit Anti-TFAP2C Antibody (clone R03-8I2) in ICC.
Immunocytochemical analysis of TFAP2C/AP2 gamma (green) in MCF-7 using TFAP2C/AP2 gamma Antibody and DAPI (blue).
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Specifications
- Immunogen
- Human AP2γ recombinant protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TFAP2C
- Species Reactivity
- Human
- Clone
- R03-8I2
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 49 kDa; Observed MW: 49 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "TFAP2C"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0643MZ | Recombinant Mouse Anti-Human TFAP2C Antibody (clone 0) | ELISA | Mouse antibody |
| VS-0923-FY150 | Recombinant Mouse Anti-TFAP2C Antibody (VS-0923-FY150) | ELISA, WB | Mouse IgG1 |
| VS13-YC1151 | Rabbit Anti-TFAP2C Recombinant Antibody (VS13-YC1151) | IHC-P | Rabbit IgG |
| VS7-0425-WR972 | Mouse Anti-TFAP2C Recombinant Antibody (clone 3A11A5) | WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3538 | Rabbit Anti-TFAP2C Recombinant Antibody (clone DS3538AB) | FC, ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2271 | Anti-TFAP2C Immunohistochemistry Kit | IHC | |
| VS-0525-XY7184 | Anti-Human TFAP2C Immunohistochemistry Kit | IHC |
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