Recombinant Mouse Anti-TUBB Antibody (VS4-CJ146), HRP
CAT#: VS4-CJ146
This mouse anti-TUBB antibody is a product that recognizes human, rat, mouse, monkey, dog, chicken, hamster, rabbit, sheep, insect, yeast TUBB. The immunogen of this antibody is a recombinant protein corresponding to TUBB. The mAb has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunohistochemistry (1:50-1:500).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5417/968_C12_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules & cytokinetic bridge.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43640/1773_E7_6_selected.jpg
Normal Tissue
Figure 3 IHC staining of human breast
Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5417/ihc_selected.jpg
Normal Tissue
Figure 4 Ovary.
High expression in ovary
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12406/31977_A_5_7_val_selected.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5417/19754_A_8_8.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12406/31977_A_9_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12406/31977_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12406/31977_A_5_6.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196230-TUBB
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Specifications
- Immunogen
- Recombinant Protein
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Sheep, Insect, Yeast TUBB
- Species Reactivity
- Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Sheep, Insect, Yeast
- Applications
- ELISA, WB, IHC
- Conjugate
- HRP
Product Property
- Purification
- Immunogen affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, PBS, pH 7.4.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunohistochemistry (1:50-1:500).
Target
- Alternative Names
- M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56
- Gene ID
- 203068
- UniProt ID
- P07437
- Long Name
- Tubulin beta class I
- Sequence Similarities
- Belongs to the tubulin family.
- Cellular Localization
- Cytoplasm, Cytoskeleton, Microtubule
- Post Translation Modifications
- Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866, PubMed:28576883).
Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Glutamylation is also involved in cilia motility (By similarity).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility (Probable) (PubMed:28576883).
Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally (Probable) (PubMed:28576883).
Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
- Protein Refseq
- NP_001280141.1; NP_001280142.1; NP_001280143.1
- Function
- Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
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- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "TUBB"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0911MZ | Recombinant Mouse Anti-Human TUBB Antibody (clone 48C) | ELISA, IHC-P, WB | Mouse antibody |
| ZG-0294U | Mouse Anti-TUBB Recombinant Antibody (clone 16E11D4) | ELISA, WB, IHC, IF, FC, IP | Mouse IgG2b |
| VS3-CJ29 | Mouse Anti-TUBB Recombinant Antibody (VS3-CJ29) | WB, ELISA | Mouse IgG |
| VS3-XY1558 | Mouse Anti-TUBB Recombinant Antibody (clone 1E1-E8-H4) | WB, ICC, IF | Mouse IgG1 |
| VS3-XY1561 | Mouse Anti-TUBB Recombinant Antibody (clone 5G3) | WB, IHC, IF |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3721 | Rabbit Anti-TUBB Recombinant Antibody (clone DS3721AB) | WB, IHC, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0816LC | Mouse Anti-TUBB Recombinant Antibody (clone TU-06) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1191 | CytoStream™ Rabbit Anti-TUBB Recombinant Antibody (VS13-YC1191) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7612 | Anti-TUBB Immunohistochemistry Kit | IHC | |
| VS-0525-XY7613 | Anti-Mouse TUBB Immunohistochemistry Kit | IHC | |
| VS-0525-XY7614 | Anti-Rat TUBB Immunohistochemistry Kit | IHC | |
| VS-0525-XY7615 | Anti-Chicken TUBB Immunohistochemistry Kit | IHC |
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