Recombinant Rabbit Anti-TUBB3 Capture Antibody (clone R19-9A-3)

CAT#: VS3-WK1808

This antibody is a rabbit recombinant antibody against human TUBB3. It was developed by immunization of rabbit with recombinant human TUBB3 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. The sandwich ELISA was established using matched antibody pair R17-2B-6 and R19-9A-3. The clone R19-9A-3 can be used as a capture antibody in ELISA analysis to identify human TUBB3.

Gene Expression
Figure 1 RNA cell line category: Cell line enhanced (A549, SCLC-21H, SH-SY5Y)

Specifications

  • Immunogen
  • Recombinant human TUBB3 protein
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human TUBB3
  • Species Reactivity
  • Human
  • Clone
  • R19-9A-3
  • Conjugate
  • Unconjugated

Product Property

  • Clonality
  • Monoclonal
  • Purification
  • Protein A affinity purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Aggregation
  • < 5%
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • 0.01M PBS, pH 7.2
  • Storage
  • Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
  • Shipping
  • Ice packs

Target

  • Alternative Names
  • Tubulin Beta 3 Class III; Class III Beta-Tubulin; Tubulin Beta-4 Chain; Tubulin Beta-III; Tubulin, Beta 3; TUBB4; Fibrosis Of Extraocular Muscles, Congenital, 3; Tubulin Beta-3 Chain
  • Long Name
  • Tubulin Beta 3 Class III
  • Sequence Similarities
  • Belongs to the tubulin family.
  • Cellular Localization
  • Cell projection, Cytoplasm, Cytoskeleton, Microtubule
  • Post Translation Modifications
  • Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group.
    Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold
  • Function
  • Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers.Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

Certificate of Analysis Lookup

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Detection antibody

Isotype Control

Secondary Antibody

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