Recombinant Rabbit Anti-UBE2N Antibody (clone R05-5B0)
CAT#: VS3-FY1515
This product is a recombinant rabbit antibody that recognizes UBE2N. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence. The clone R05-5B0 is specific for human, rat UBE2N. The antigen is synthetic peptide of human ube2n/ubc13.
Tested Data
Gene Expression
WB
Figure 1. Recombinant Rabbit Anti-UBE2N Antibody (clone R05-5B0) in WB.
Western Blot analysis of Ube2N/Ubc13 in HL-60, C6 lysates using Ube2N/Ubc13 Antibody.
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Subcellular Location
Figure 1 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44976/1422_E11_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44976/113074_B_7_5.jpg
Normal Tissue
Figure 3 Stomach
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44976/113074_A_5_1.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44976/113074_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44976/113074_A_9_5.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000177889-UBE2N
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Specifications
- Immunogen
- Synthetic peptide of human Ube2N/Ubc13.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat UBE2N
- Species Reactivity
- Human, Rat
- Clone
- R05-5B0
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 17 kDa; Observed MW: 17 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Ubiquitin Conjugating Enzyme E2 N; Ubiquitin-Conjugating Enzyme E2N (Homologous To Yeast UBC13); Ubiquitin-Conjugating Enzyme E2N (UBC13 Homolog, Yeast); E2 Ubiquitin-Conjugating Enzyme N; Ubiquitin Carrier Protein N; Ubiquitin-Protein Ligase N; UbcH13; UBC13; Bendless-Like Ubiquitin Conjugating Enzyme; Bendless-Like Ubiquitin-Conjugating Enzyme; Epididymis Secretory Protein Li 71
- Gene ID
- 7334
- UniProt ID
- P61088
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "UBE2N"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3760 | Rabbit Anti-UBE2N Recombinant Antibody (clone DS3760AB) | FC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1550-CN | Rabbit Anti-UBE2N Recombinant Antibody (clone JG38-68) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1202 | Rabbit Anti-UBE2N Recombinant Antibody (VS13-YC1202) | WB, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2418 | Anti-UBE2N Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7704 | Anti-Human UBE2N Immunohistochemistry Kit | IHC |
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