What is the main application of this antibody?

This antibody is mostly used for Western Blot, Immunohistochemistry in frozen or paraffin sections, Immunocytochemistry, and Immunofluorescence. It is helpful in detecting VPS4A in various species.

Which species can this antibody be used with?

The antibody can detect VPS4A in humans, mice, and rats.

What is VPS4A and why is it important?

VPS4A is a vacuolar protein involved in sorting and is essential in cellular transport mechanisms. It plays a key role in endosomal sorting complexes required for transport and has implications in understanding cell biology.

Recombinant Rabbit Anti-VPS4A Antibody (clone R01-9H9) (CAT#: VS3-FY1548)

This product is a recombinant rabbit antibody that recognizes VPS4A. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R01-9H9 is specific for human, mouse, rat VPS4A. The antigen is synthetic peptide of human vps4a.

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Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1. Recombinant Rabbit Anti-VPS4A Antibody (clone R01-9H9) in WB.

Figure 1. Recombinant Rabbit Anti-VPS4A Antibody (clone R01-9H9) in WB.

Western Blot analysis of VPS4a in K562, rat brain, C6, 3T3 lysates using VPS4A Antibody.

Subcellular Location

Figure 1 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to midbody.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60092/1184_E11_4_selected.jpg

Normal Tissue

Figure 2 IHC staining of human appendix
(Immunohistochemical staining of human appendix shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18751/ihc_selected.jpg

Figure 3 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34411/127437_A_7_8.jpg

Figure 4 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34411/127437_B_9_5.jpg

Figure 5 Colon
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34411/127437_A_9_3.jpg

Figure 6 Kidney
(Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34411/127437_A_8_5.jpg

Figure 7 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34411/127437_A_6_6.jpg

Figure 8 Ovary
(Follicle cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Ovarian stroma cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34411/127437_A_5_7.jpg

RNA Expression

Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132612-VPS4A

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Synthetic peptide of human VPS4a.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Mouse, Rat VPS4A

Species Reactivity
Human, Mouse, Rat

Clone
R01-9H9

Applications
Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence

Conjugate
Unconjugated

MW
Calculated MW: 49 kDa; Observed MW: 49 kDa

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200

Target

Alternative Names
Vacuolar Protein Sorting 4 Homolog A; VPS4-1; HVPS4; VPS4; Vacuolar Protein Sorting 4 Homolog A (S. Cerevisiae); Vacuolar Protein Sorting-Associated Protein 4A; Vacuolar Protein Sorting 4A (Yeast Homolog); Vacuolar Protein Sorting 4A (Yeast); Vacuolar Protein Sorting Factor 4A

Gene ID
27183

UniProt ID
Q9UN37

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Rabbit Monoclonal Antibody Products

CAT	Product Name	Application	Type
MOR-3851	Rabbit Anti-VPS4A Recombinant Antibody (clone DS3851AB)	WB, ICC, IF, FC, IHC-P	Rabbit IgG

Customer Reviews and Q&As

Customer Review Q&As

Great for Multiple Applications

The antibody is very versatile and works well for Western Blot and Immunocytochemistry. I used it for my human cell line studies, and the results were clear and consistent.

Perfect for Immunofluorescence

This antibody was ideal for my immunofluorescence experiments. The fluorescence was bright and specific, enhancing the visualization of VPS4A in my samples.

Strong Immunohistochemistry Results

I used this antibody on paraffin sections, and it performed excellently. The staining was very strong, which aided in my research on DVD4-VPS41 interactions.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# VS3-FY1548, RRID: AB_3112059)

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.