Recombinant Rabbit Anti-VPS4A Antibody (clone R01-9H9)
CAT#: VS3-FY1548
This product is a recombinant rabbit antibody that recognizes VPS4A. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R01-9H9 is specific for human, mouse, rat VPS4A. The antigen is synthetic peptide of human vps4a.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to midbody.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60092/1184_E11_4_selected.jpg
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WB
Figure 1. Recombinant Rabbit Anti-VPS4A Antibody (clone R01-9H9) in WB.
Western Blot analysis of VPS4a in K562, rat brain, C6, 3T3 lysates using VPS4A Antibody.
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Specifications
- Immunogen
- Synthetic peptide of human VPS4a.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat VPS4A
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-9H9
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 49 kDa; Observed MW: 49 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Vacuolar Protein Sorting 4 Homolog A; VPS4-1; HVPS4; VPS4; Vacuolar Protein Sorting 4 Homolog A (S. Cerevisiae); Vacuolar Protein Sorting-Associated Protein 4A; Vacuolar Protein Sorting 4A (Yeast Homolog); Vacuolar Protein Sorting 4A (Yeast); Vacuolar Protein Sorting Factor 4A
- Gene ID
- 27183
- UniProt ID
- Q9UN37
REVIEWS AND Q&AS
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Customer Review
There are currently no Customer reviews or questions for VS3-FY1548. Click the button above to contact us or submit your feedback about this product.
Great for Multiple Applications
The antibody is very versatile and works well for Western Blot and Immunocytochemistry. I used it for my human cell line studies, and the results were clear and consistent.
Perfect for Immunofluorescence
This antibody was ideal for my immunofluorescence experiments. The fluorescence was bright and specific, enhancing the visualization of VPS4A in my samples.
Strong Immunohistochemistry Results
I used this antibody on paraffin sections, and it performed excellently. The staining was very strong, which aided in my research on DVD4-VPS41 interactions.
Q&As
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What is the main application of this antibody?
A: This antibody is mostly used for Western Blot, Immunohistochemistry in frozen or paraffin sections, Immunocytochemistry, and Immunofluorescence. It is helpful in detecting VPS4A in various species.
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Which species can this antibody be used with?
A: The antibody can detect VPS4A in humans, mice, and rats.
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What is VPS4A and why is it important?
A: VPS4A is a vacuolar protein involved in sorting and is essential in cellular transport mechanisms. It plays a key role in endosomal sorting complexes required for transport and has implications in understanding cell biology.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# VS3-FY1548, RRID: AB_3112059)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "VPS4A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3851 | Rabbit Anti-VPS4A Recombinant Antibody (clone DS3851AB) | WB, ICC, IF, FC, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7870 | Anti-VPS4A Immunohistochemistry Kit | IHC |
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