Recombinant Rabbit Anti-VPS4A Antibody (clone R01-9H9)

CAT#: VS3-FY1548

This product is a recombinant rabbit antibody that recognizes VPS4A. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R01-9H9 is specific for human, mouse, rat VPS4A. The antigen is synthetic peptide of human vps4a.

Gene Expression Tested Data
Figure 1 IF staining of human cell line SiHa Figure 2 IHC staining of human appendix Figure 3 Lymph node Figure 4 Cerebral cortex Figure 5 Colon Figure 6 Kidney Figure 7 Testis Figure 8 Ovary Figure 9 RNA cell line category: Low cell line specificity
Figure 1. Recombinant Rabbit Anti-VPS4A Antibody (clone R01-9H9) in WB.

Specifications

  • Immunogen
  • Synthetic peptide of human VPS4a.
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse, Rat VPS4A
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R01-9H9
  • Applications
  • Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
  • Conjugate
  • Unconjugated
  • MW
  • Calculated MW: 49 kDa; Observed MW: 49 kDa

Product Property

  • Purification
  • Affinity Purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry-Paraffin: 1/50-1/100
    Immunofluorescence: 1/50-1/200

Target

  • Alternative Names
  • Vacuolar Protein Sorting 4 Homolog A; VPS4-1; HVPS4; VPS4; Vacuolar Protein Sorting 4 Homolog A (S. Cerevisiae); Vacuolar Protein Sorting-Associated Protein 4A; Vacuolar Protein Sorting 4A (Yeast Homolog); Vacuolar Protein Sorting 4A (Yeast); Vacuolar Protein Sorting Factor 4A
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Breast cancer biomarkers at key points during disease progression
Great for Multiple Applications
The antibody is very versatile and works well for Western Blot and Immunocytochemistry. I used it for my human cell line studies, and the results were clear and consistent.
Breast cancer biomarkers at key points during disease progression
Perfect for Immunofluorescence
This antibody was ideal for my immunofluorescence experiments. The fluorescence was bright and specific, enhancing the visualization of VPS4A in my samples.
Breast cancer biomarkers at key points during disease progression
Strong Immunohistochemistry Results
I used this antibody on paraffin sections, and it performed excellently. The staining was very strong, which aided in my research on DVD4-VPS41 interactions.

Q&As

  1. What is the main application of this antibody?

    A: This antibody is mostly used for Western Blot, Immunohistochemistry in frozen or paraffin sections, Immunocytochemistry, and Immunofluorescence. It is helpful in detecting VPS4A in various species.

  2. Which species can this antibody be used with?

    A: The antibody can detect VPS4A in humans, mice, and rats.

  3. What is VPS4A and why is it important?

    A: VPS4A is a vacuolar protein involved in sorting and is essential in cellular transport mechanisms. It plays a key role in endosomal sorting complexes required for transport and has implications in understanding cell biology.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# VS3-FY1548, RRID: AB_3112059)

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Secondary Antibody

Isotype Control

See other products for "VPS4A"

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MOR-3851 Rabbit Anti-VPS4A Recombinant Antibody (clone DS3851AB) WB, ICC, IF, FC, IHC-P Rabbit IgG
CAT Product Name Application Type
VS-0525-XY7870 Anti-VPS4A Immunohistochemistry Kit IHC
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