Recombinant Rabbit Anti-YY1 Antibody (clone R01-1D3)
CAT#: VS3-FY1571
This product is a recombinant rabbit antibody that recognizes YY1. This antibody has been reported for use in Western Blot, Immunoprecipitation. The clone R01-1D3 is specific for human, mouse, rat YY1. The antigen is human yy1 synthetic peptide.
Tested Data
WB
Figure 1. Recombinant Rabbit Anti-YY1 Antibody (clone R01-1D3) in WB.
Western Blot analysis of YY1 in C6, 3T3, Hela lysates using YY1 antibody.
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Specifications
- Immunogen
- Human YY1 synthetic peptide.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat YY1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-1D3
- Applications
- Western Blot, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 45 kDa; Observed MW: 65 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- YY1 Transcription Factor; INO80 Complex Subunit S; Delta Transcription Factor; Yin And Yang 1 Protein; INO80S; NF-E1; YY-1
- Gene ID
- 7528
- UniProt ID
- P25490
- Sequence Similarities
- Belongs to the YY transcription factor family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylation at Ser-118 by CK2 prevents proteolytic cleavage by caspase-7 (CASP7) during apoptosis.
Proteolytically cleaved by caspase-7 (CASP7) in response to apoptosis (PubMed:22184066).
Phosphorylation at Ser-118 protects against proteolytic cleavage (PubMed:22184066).
Transiently poly-ADP-ribosylated by PARP1 upon DNA damage, with the effect of decreasing affinity of YY1 to its cognate DNA binding sites.
Ubiquitinated.
- Protein Refseq
- NP_003394.1
- Function
- Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (PubMed:15329343). Binds to SMAD binding elements (SBEs) (5'- GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions. May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions).
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Related Signaling Pathways
mTOR Signaling Pathway
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "YY1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2115CT | Recombinant Mouse anti-Human YY1 Monoclonal antibody (EML2147) | IHC-P, WB | |
| MRO-1609-CN | Rabbit Anti-YY1 Recombinant Antibody (clone CBACN-587) | WB, IF, IHC, IP | Rabbit IgG |
| ZG-0624U | Rabbit Anti-YY1 Recombinant Antibody (clone 3A11) | ELISA | Rabbit IgG |
| VS3-CJ821 | Mouse Anti-YY1 Recombinant Antibody (VS3-CJ821) | WB, IHC | Mouse IgG2a |
| VS3-CJ822 | Rabbit Anti-YY1 Recombinant Antibody (VS3-CJ822) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3909 | Rabbit Anti-YY1 Recombinant Antibody (clone DS3909AB) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2515 | Anti-YY1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7997 | Anti-Human YY1 Immunohistochemistry Kit | IHC |
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