Recombinant Rabbit Anti-YY1 Antibody (clone R01-1D3) (CAT#: VS3-FY1571)

• Immunogen
• Human YY1 synthetic peptide.

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse, Rat YY1

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R01-1D3

• Applications
• Western Blot, Immunoprecipitation

• Conjugate
• Unconjugated

• MW
• Calculated MW: 45 kDa; Observed MW: 65 kDa

• Purification
• Affinity Purified

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunoprecipitation: 1/20

• Alternative Names
• YY1 Transcription Factor; INO80 Complex Subunit S; Delta Transcription Factor; Yin And Yang 1 Protein; INO80S; NF-E1; YY-1

• Gene ID
• 7528

• UniProt ID
• P25490

• Sequence Similarities
• Belongs to the YY transcription factor family.

• Cellular Localization
• Nucleus

• Post Translation Modifications
• Phosphorylation at Ser-118 by CK2 prevents proteolytic cleavage by caspase-7 (CASP7) during apoptosis.
  Proteolytically cleaved by caspase-7 (CASP7) in response to apoptosis (PubMed:22184066).
  Phosphorylation at Ser-118 protects against proteolytic cleavage (PubMed:22184066).
  Transiently poly-ADP-ribosylated by PARP1 upon DNA damage, with the effect of decreasing affinity of YY1 to its cognate DNA binding sites.
  Ubiquitinated.

• Protein Refseq
• NP_003394.1

• Function
• Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (PubMed:15329343). Binds to SMAD binding elements (SBEs) (5'- GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions. May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions).

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-060CQ
• Llama Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody