Ankyrin repeat scaffold protein anti-Human PDGFB
CAT#: SAR-L042
The Ankyrin repeat that bound PDGFB was isolated from the library using ribosome display techonology. The ankyrin repeat is a 33-residue motif in proteins consisting of two alpha helices separated by loops, first discovered in signaling proteins in yeast Cdc10 and Drosophila Notch. Domains consisting of ankyrin repeats mediate protein–protein interactions and are among the most common structural motifs in known proteins. After affinity maturation, Ankyrin repeat variants were identified having affinities (KD=1.5 nM) for PDGFB as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11972/1592_D1_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human caudate
Immunohistochemical staining of human caudate shows strong cytoplasmic positivity in granular pattern in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11972/ihc_selected.jpg
Normal Tissue
Figure 3 Placenta
Cytotrophoblasts Staining: Medium Intensity: Moderate Quantity:>75% Endothelial cells Staining: High Intensity: Strong Quantity:>75% Hofbauer cells Staining: Medium Intensity: Moderate Quantity:>75% Syncytiotrophoblasts - cell body Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - microvilli Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11972/28202_A_1_7.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11972/28202_A_9_8.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11972/28202_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11972/28202_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18341/40769_A_5_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (A-431, EFO-21, HUVEC TERT2, SK-BR-3, TIME)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100311-PDGFB
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Specifications
- Scaffold Name
- Ankyrin repeat
- Origin
- Ankyrin repeat proteins
- Core Structure
- 2α-helices, β-turn
- Variable Regions
- b-turn,1a-helix and loop
- Target
- PDGFB
- Species Reactivity
- Human
- Expression Host
- E. coli
- Affinity Constant
- 1.5 nM
- Applications
- ELISA; immunohistochemistry; Microscopy; Flow Cytometer; Western Blotting; Functional Study
Target
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| HPAB-0387-CN-S(P) | Rat Anti-PDGFB Recombinant Antibody; scFv Fragment (HPAB-0387-CN-S(P)) | ELISA, Block | Rat scFv |
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| CAT | Product Name | Application | Type |
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| PFBL-297 | Human Anti-PDGFB Recombinant Antibody Fab Fragment (PFBL-297) | WB, ELISA, FuncS | Human Fab |
| HPAB-0385-CN-F(E) | Human Anti-PDGFB Recombinant Antibody; Fab Fragment (HPAB-0385-CN-F(E)) | ELISA, FC, IF, Block | Human Fab |
| HPAB-0386-CN-F(E) | Rat Anti-PDGFB Recombinant Antibody; Fab Fragment (HPAB-0386-CN-F(E)) | ELISA, Block | Rat Fab |
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| CAT | Product Name | Application | Type |
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| TAB-1021CL | Anti-Human PDGFB Recombinant Antibody (C12) | ELISA | Humanized antibody |
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| TAB-1021CL-F(E) | Anti-Human PDGFB Recombinant Antibody Fab Fragment (C12) | ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2635 | Hi-Affi™ Recombinant Rabbit Anti-PDGFB Monoclonal Antibody (DS2635AB) | WB, IHC-P | IgG |
| CAT | Product Name | Application | Type |
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| VS-0525-XY5273 | Anti-PDGFB Immunohistochemistry Kit | IHC |
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