Hi-Affi™ Rabbit Anti-ACTB Recombinant Antibody (clone DS49AB)
CAT#: MOR-0049
Recombinant Rabbit anti-ACTB monoclonal antibody for IHC-P, WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2621/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_B_7_5.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2621/6575_A_6_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_7_8.jpg
Normal Tissue
Figure 5 Colon
Enterocytes - Microvilli Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_8_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_9_5.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000075624-ACTB
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Specifications
- Immunogen
- Synthetic peptide near the N-terminus of Human beta Actin.
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- Rabbit IgG
- Species Reactivity
- Human
- Clone
- DS49AB
- Applications
- IHC-P, WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "ACTB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3039z | Mouse Anti-ACTB Recombinant Antibody (clone 28D10) | WB, ELISA, ICC, IF | Mouse IgG1, κ |
| MRO-0170-CN | Rabbit Anti-ACTB Recombinant Antibody (clone JF53-10) | WB | Rabbit IgG |
| MRO-0174-CN | Mouse Anti-ACTB Recombinant Antibody (clone A2-F6) | WB, IF, IHC, FC | Mouse IgG1 |
| ZG-0296U | Mouse Anti-ACTB Recombinant Antibody (clone 1D12E12B5) | ELISA, WB, IHC, IF, FC, IP | Mouse IgG2b |
| VS-0923-FY1 | Recombinant Mouse Anti-ACTB Antibody (VS-0923-FY1) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-055 | Recombinant Anti-human ACTB VHH Single Domain Antibody | WB, ELISA, IP, FC, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0345MC | Recombinant Human Anti-Actin Beta Antibody Fab Fragment | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0017MZ | Chicken Anti-Actin, Cytoplasmic 1 Polyclonal IgY | WB | Chicken antibody |
| BRD-0018MZ | Chicken Anti-Actin, Non-muscle (β and γ) Polyclonal IgY | WB | Chicken antibody |
| BRD-0687MZ | Chicken Anti-Beta actin Polyclonal IgY | ICC, IF, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0283U | Mouse Anti-ACTB Recombinant Antibody (ZG-0283U) | ELISA | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1253 | AbPlus™ Anti-ACTB Magnetic Beads (VS-0724-YC1253) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY53 | Mouse Anti-NHP ACTB Recombinant Antibody (VS-1024-XY53) | WB, IHC | Mouse IgG2b, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY28 | Anti-ACTB Immunohistochemistry Kit | IHC | |
| VS-0525-XY114 | Anti-Mouse ACTB Immunohistochemistry Kit | IHC | |
| VS-0525-XY113 | Anti-Human ACTB Immunohistochemistry Kit | IHC | |
| VS-0525-XY115 | Anti-Rat ACTB Immunohistochemistry Kit | IHC |
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