Anti-Human ACTB Immunohistochemistry Kit
CAT#: VS-0525-XY113
The ACTB IHC kit provides strong and reliable detection of beta-actin, widely used as a housekeeping marker. With complete reagents and compatibility with both tissue types, it's perfect for normalization and cytoskeletal studies.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2621/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_B_7_5.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2621/6575_A_6_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_7_8.jpg
Normal Tissue
Figure 5 Colon
Enterocytes - Microvilli Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_8_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_9_5.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000075624-ACTB
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Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human
- Target
- ACTB
- Primary Antibody
- Rabbit Anti-ACTB Antibody
- Secondary Antibody
- Goat anti-Rabbit Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| MOB-1593MZ | Recombinant Mouse Anti-Human ACTB Antibody | IHC-P, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
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| BRD-0017MZ | Chicken Anti-Actin, Cytoplasmic 1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
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| BRD-0018MZ | Chicken Anti-Actin, Non-muscle (β and γ) Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| MOR-0049 | Hi-Affi™ Rabbit Anti-ACTB Recombinant Antibody (clone DS49AB) | IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| MHC-LC3876 | PE-A*02:01/Human ACTB (GMGQKDSYV) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC3877 | PE-A*02:03/Human ACTB (GMGQKDSYV) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC3878 | PE-A*02:02/Human ACTB (GMGQKDSYV) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC3879 | PE-A*02:02/Human ACTB (AMYVAIQAV) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC3880 | PE-A*02:03/Human ACTB (AMYVAIQAV) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0085-CN | Mouse Anti-ACTB Recombinant Antibody (clone A5-E6) | WB, IF, IHC, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0170-CN | Rabbit Anti-ACTB Recombinant Antibody (clone JF53-10) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0174-CN | Mouse Anti-ACTB Recombinant Antibody (clone A2-F6) | WB, IF, IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0283U | Mouse Anti-ACTB Recombinant Antibody (ZG-0283U) | ELISA | Mouse IgM |
| CAT | Product Name | Application | Type |
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| VS-0724-YC1253 | AbPlus™ Anti-ACTB Magnetic Beads (VS-0724-YC1253) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY53 | Mouse Anti-NHP ACTB Recombinant Antibody (VS-1024-XY53) | WB, IHC | Mouse IgG2b, kappa |
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| VS-0325-XY28 | Anti-ACTB Immunohistochemistry Kit | IHC |
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| VS-0525-XY114 | Anti-Mouse ACTB Immunohistochemistry Kit | IHC |
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| VS-0525-XY115 | Anti-Rat ACTB Immunohistochemistry Kit | IHC |
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