The antibodies bind to the second extracellular loop of the human β1-adrenoreceptor (β1-AR-ECII). The antibodies are particularly useful in determination of auto-anti-β1-AR antibodies in an in vitro cell based assay system in order to characterize and to identify auto-antibodies directed against the β1-AR-ECII in a biological sample.
Figure 1 Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3121) (HPAB-0273-YC) in SDS-PAGE
SDS-PAGE analysis of HPAB-0273-YC in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
Figure 2 Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3121) (HPAB-0273-YC) in SEC-HPLC
The purity of this antibody is> 95% as determined by SEC-HPLC.
Figure 3 Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3121) (HPAB-0273-YC) in WB
Western blot analysis of HPAB-0273-YC was performed by loading ADRB1 peptide (HWWRAESDEARRCYNDPKCCDFVTNR-BSA).
HPAB-0273-YC incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat anti-Mouse IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
Figure 4 Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3121) (HPAB-0273-YC) in ELISA
ELISA analysis of HPAB-0273-YC was performed by coating with ADRB1 peptide (HWWRAESDEARRCYNDPKCCDFVTNR-BSA).
The secondary antibody: HRP-Goat anti-Mouse IgG
Figure 5 Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3121) (HPAB-0273-YC) in DB
Dot Blot analysis of HPAB-0273-YC was performed by coating with ADRB1 peptide (HWWRAESDEARRCYNDPKCCDFVTNR-BSA).
The secondary antibody: HRP-Goat anti-Mouse IgG
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
NAB-114-VHH | Recombinant Anti-Human ADRB1 VHH Single Domain Antibody | ICC, ChiP, FC, FA | Llama VHH |
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-0273-YC, RRID: AB_3111378)
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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