Mouse Anti-ATP2A1 Recombinant Antibody (clone 7B2)
CAT#: MOB-2405CT
Recombinant Mouse Monoclonall antibody to Human ATP2A1, expressed in mammalian cells.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2310/if_selected.jpg
Normal Tissue
Figure 2 High expression in skeletal muscle.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32706/ihc_selected.jpg
Normal Tissue
Figure 3 Low expression in heart muscle.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32706/128663_B_5_6_val_selected.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32706/128663_A_9_5.jpg
Normal Tissue
Figure 5 Skeletal muscle
Myocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32706/128663_B_1_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HEK93)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000196296-ATP2A1
❮
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Specifications
- Immunogen
- Recombinant fragment: IDRCNYVRVG TTRVPLTGPV KEKIMAVIKE WGTGRDTLRC LALATRDTPP KREEMVLDDS ARFLEYETDL TFVGVVGMLD PPRKEVTGSI QL, corresponding to amino acids 522-613 of Human SERCA1 ATPasewith a proprietary tag.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Skeletal muscle, fast twitch muscle (type II) fibers.
- Species Reactivity
- Human
- Clone
- 7B2
- Applications
- WB, ELISA, IHC-P, IF
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Buffer
- Preservative: None. Constituents: PBS, pH 7.2
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- ATP2A1; ATPase, Ca++ transporting, cardiac muscle, fast twitch 1; ATP2A; SERCA1; sarcoplasmic/endoplasmic reticulum calcium ATPase 1; calcium pump 1; SR Ca(2+)-ATPase 1; endoplasmic reticulum class 1/2 Ca(2+) ATPase; calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform;
- Gene ID
- 487
- UniProt ID
- O14983
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Related Signaling Pathways
cAMP Signaling Pathway
Related Diseases
Morphine Addiction
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| VS-0525-XY611 | Anti-ATP2A1 Immunohistochemistry Kit | IHC |
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