Recombinant Llama Anti-BMP4 Single Domain Antibody (HPAB-0355-YC-VHH) (CAT#: HPAB-0355-YC-VHH)
Provided are anti-BMP4 antibodies binding to a conformational epitope located within the BMPRla binding site of BMP4 and pharmaceutical uses thereof.
Specific Inquiry
Figure 1 Anti-BMP4 VHH specificity to the different BMPs.
Phosphorylation of SMAD1/5/8 in EPC2-hERT cells after activation with 100 ng/mL of human BMP4 in the presence of 5 mg/mL VHHs.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
FuncS
Figure 2 Anti-BMP4 VHH specificity to the different BMPs.
C2C12 cells were activated with human BMPs in the presence or absence of 5 mg/mL of VHHs or human Fc-Noggin.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
Activ
Figure 3 IC50 curves of VHH biheads and monoheads.
C2C12 cells were activated with human BMP4 at 5 ng/mL with increasing concentrations of VHHs.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
SPR
Figure 4 Kinetic analysis for BMP binding.
Binding of VHHs to the different BMPs was tested in a direct SPR assay.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
SPR
Figure 5 Kinetic analysis for BMP binding.
Epitope binning of VHH-coated chip plates. BMP4 (1.0 mg/mL) was injected on the chip followed by the injection of a second VHH (2.0 mg/mL) or the corresponding controls.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
FuncS
Figure 6 HADDOCK docking of the VHHs to BMP4 binding.
Function of the generated mutant variants in inhibiting ID1 promoter activity in C2C12 cells after stimulation with BMP4 (5 ng/mL) for 16 hours.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
Specifications
- Immunogen
- BMP4
- Host Species
- Llama
- Derivation
- The VHH library
- Type
- Llama VHH
- Specificity
- Human BMP4
- Species Reactivity
- Human
- Clone
- HPAB-0355-YC-VHH
- Applications
- Functional Assay, Surface plasmon resonance, Activation
- Related Disease
- Colorectal cancer
Applications
- Application Notes
- The antibody was validated for Functional Assay, Surface plasmon resonance, Activation. For details, refer to Published Data.
Target
- Alternative Names
- Bone Morphogenetic Protein 4; Bone Morphogenetic Protein 2B; BMP-2B; BMP2B; BMP-4; BMP2B1; MCOPS6; OFC11; Zyme; DVR4
- Gene ID
- 652
- UniProt ID
- P12644
Related Resources
TGF-β Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2244z | Mouse Anti-BMP4 Recombinant Antibody (clone 18H6) | WB, ELISA, FC, ICC, IHC | Mouse IgG1 |
| ZG-0339F | Mouse Anti-BMP4 Recombinant Antibody (ZG-0339F) | WB, ELISA | Mouse IgG |
| VS3-QX123 | Mouse Anti-BMP4 Recombinant Antibody (clone 1C-17) | WB, ICC, FC | Mouse IgG1 |
| VS3-QX124 | Mouse Anti-BMP4 Recombinant Antibody (clone 10F4B4) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS4-WK50 | Rabbit Anti-BMP4 Recombinant Antibody (clone 19B7) | ELISA, WB, IF | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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