Recombinant Llama Anti-BMP4 Single Domain Antibody (HPAB-0355-YC-VHH)
CAT#: HPAB-0355-YC-VHH
Provided are anti-BMP4 antibodies binding to a conformational epitope located within the BMPRla binding site of BMP4 and pharmaceutical uses thereof.
Published Data
Gene Expression
Figure 1 Anti-BMP4 VHH specificity to the different BMPs.
Phosphorylation of SMAD1/5/8 in EPC2-hERT cells after activation with 100 ng/mL of human BMP4 in the presence of 5 mg/mL VHHs.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
FuncS
Figure 2 Anti-BMP4 VHH specificity to the different BMPs.
C2C12 cells were activated with human BMPs in the presence or absence of 5 mg/mL of VHHs or human Fc-Noggin.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
Activ
Figure 3 IC50 curves of VHH biheads and monoheads.
C2C12 cells were activated with human BMP4 at 5 ng/mL with increasing concentrations of VHHs.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
SPR
Figure 4 Kinetic analysis for BMP binding.
Binding of VHHs to the different BMPs was tested in a direct SPR assay.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
SPR
Figure 5 Kinetic analysis for BMP binding.
Epitope binning of VHH-coated chip plates. BMP4 (1.0 mg/mL) was injected on the chip followed by the injection of a second VHH (2.0 mg/mL) or the corresponding controls.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
FuncS
Figure 6 HADDOCK docking of the VHHs to BMP4 binding.
Function of the generated mutant variants in inhibiting ID1 promoter activity in C2C12 cells after stimulation with BMP4 (5 ng/mL) for 16 hours.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
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Normal Tissue
Figure 1 IHC staining of human smooth muscle
Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/66235/ihc_selected.jpg
Normal Tissue
Figure 2 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows distinct cytoplasmic and extra cellular positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/6863/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/66235/153903_A_7_3.jpg
Normal Tissue
Figure 4 Smooth muscle
Smooth muscle cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/66235/153903_B_6_7.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (CACO-2, CAPAN-2, Hep G2, OE19, SuSa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000125378-BMP4
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Specifications
- Immunogen
- BMP4
- Host Species
- Llama
- Derivation
- The VHH library
- Type
- Llama VHH
- Specificity
- Human BMP4
- Species Reactivity
- Human
- Clone
- HPAB-0355-YC-VHH
- Applications
- Functional Assay, Surface plasmon resonance, Activation
- Related Disease
- Colorectal cancer
Applications
- Application Notes
- The antibody was validated for Functional Assay, Surface plasmon resonance, Activation. For details, refer to Published Data.
Target
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Related Signaling Pathways
TGF-β Signaling Pathway
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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MSDS
COA
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