Recombinant Llama Anti-BMP4 Single Domain Antibody (HPAB-0356-YC-VHH)
CAT#: HPAB-0356-YC-VHH
For several years the role of specific BMPs has been addressed in many nondiseased and diseased tissues. One of the most intriguing and poorly understood BMPs is BMP4. BMP4 seems to have opposing roles in cancers.
Published Data
Gene Expression
FuncS
Figure 1 IC50 curves of VHH biheads and monoheads.
C2C12 cells were activated with human BMP4 at 5 ng/mL with increasing concentrations of VHHs.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
SPR
Figure 2 Kinetic analysis for BMP binding.
Binding of VHHs to the different BMPs was tested in a direct SPR assay. The graphs show two replicate ligand injections at just one concentration of ligand (0.4 mg/mL for BMP4).
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
SPR
Figure 3 Kinetic analysis for BMP binding.
Epitope binning of VHH-coated chip plates. BMP4 (1.0 mg/mL) was injected on the chip followed by the injection of a second VHH (2.0 mg/mL) or the corresponding controls.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
SPR
Figure 4 Kinetic analysis for BMP binding.
Epitope binning of VHH-coated chip plates. BMP4 (1.0 mg/mL) was injected on the chip followed by the injection of a second VHH (2.0 mg/mL) or the corresponding controls.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
Inhib
Figure 5 HADDOCK docking of the VHHs to BMP4 binding.
Function of the generated mutant variants in inhibiting ID1 promoter activity in C2C12 cells after stimulation with BMP4 (5 ng/mL) for 16 hours. Uns, unstimulated C2C12 cells. Values calculated from 3 independent experiments, with experimental triplicates each.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
❮
❯
Normal Tissue
Figure 1 IHC staining of human smooth muscle
Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/66235/ihc_selected.jpg
Normal Tissue
Figure 2 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows distinct cytoplasmic and extra cellular positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/6863/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/66235/153903_A_7_3.jpg
Normal Tissue
Figure 4 Smooth muscle
Smooth muscle cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/66235/153903_B_6_7.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (CACO-2, CAPAN-2, Hep G2, OE19, SuSa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000125378-BMP4
❮
❯
Specifications
- Host Species
- Llama
- Derivation
- The VHH library
- Type
- Llama VHH
- Specificity
- Human BMP4
- Species Reactivity
- Human
- Clone
- HPAB-0356-YC-VHH
- Applications
- Inhibition, Surface plasmon resonance
- Related Disease
- Colorectal cancer
Applications
- Application Notes
- The antibody was validated for Inhibition, Surface plasmon resonance. For details, refer to Published Data.
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for HPAB-0356-YC-VHH. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Signaling Pathways
TGF-β Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "BMP4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2244z | Mouse Anti-BMP4 Recombinant Antibody (clone 18H6) | WB, ELISA, FC, ICC, IHC | Mouse IgG1 |
| VS3-QX123 | Mouse Anti-BMP4 Recombinant Antibody (clone 1C-17) | WB, ICC, FC | Mouse IgG1 |
| VS3-QX124 | Mouse Anti-BMP4 Recombinant Antibody (clone 10F4B4) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS7-HM242 | Mouse Anti-BMP4 Recombinant Antibody (clone 3C11H8) | WB, ICC | Mouse IgG1 |
| VS7-HM243 | Mouse Anti-BMP4 Recombinant Antibody (clone 3C11C7) | WB, ICC, FCM | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1272CL | Mouse Anti-BMP4 Recombinant Antibody (TAB-1272CL) | FuncS | Mouse IgG2b, κ |
| TAB-1272CL-S(P) | Mouse Anti-BMP4 Recombinant Antibody; scFv Fragment (TAB-1272CL-S(P)) | FuncS | Mouse scFv |
| TAB-1273CL-S(P) | Mouse Anti-BMP4 Recombinant Antibody; scFv Fragment (TAB-1273CL-S(P)) | FuncS | Mouse scFv |
| TAB-1272CL-F(E) | Mouse Anti-BMP4 Recombinant Antibody; Fab Fragment (TAB-1272CL-F(E)) | FuncS | Mouse Fab |
| TAB-1273CL-F(E) | Mouse Anti-BMP4 Recombinant Antibody; Fab Fragment (TAB-1273CL-F(E)) | FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0069CL-VHH | Anti-Human BMP4 VHH Single Domain Antibody (TAB-0069CL-VHH) | ELISA, WB | Single domain antibody (VHH) |
| HPAB-0355-YC-VHH | Recombinant Llama Anti-BMP4 Single Domain Antibody (HPAB-0355-YC-VHH) | FuncS, SPR, Activ | Llama VHH |
| HPAB-0357-YC-VHH | Recombinant Llama Anti-BMP4 Single Domain Antibody (HPAB-0357-YC-VHH) | Inhib, SPR | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-051CQ | Mouse Anti-BMP4 Recombinant Antibody (clone 3H2) | ELISA, Neut, WB | Mouse IgG2b, κ |
| NEUT-052CQ | Mouse Anti-BMP4 Recombinant Antibody (clone CBL815) | Neut, WB | Mouse IgG2b |
| NEUT-053CQ | Mouse Anti-BMP4 Recombinant Antibody (clone CBL816) | WB, Neut, ELISA | Mouse IgG2b |
| NEUT-054CQ | Mouse Anti-BMP4 Recombinant Antibody (clone CBL987) | ELISA, WB, IHC, Neut | Mouse IgG2b, κ |
| NEUT-055CQ | Rat Anti-BMP4 Recombinant Antibody (clone CBL387) | Neut | Rat IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0367 | Hi-Affi™ Rabbit Anti-BMP4 Recombinant Antibody (clone DS367AB) | WB, IHC, ICC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY251 | Anti-BMP4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY800 | Anti-Rat BMP4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY801 | Anti-Mouse BMP4 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC31 | Recombinant Anti-BMP4 Eliminating Antibody, pH-Sensitive (VS-0625-YC31) | Antigen-Sweeping In Vivo. | Mouse IgG2b kappa |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: ELISA, WB, BLI, SPR
Application: WB, ELISA, FuncS, Inhib, PK, IP, SPR
Application: ELISA, FC
Application: Neut, FuncS, IHC, ELISA, FC, Inhib
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
