Recombinant Llama Anti-BMP4 Single Domain Antibody (HPAB-0357-YC-VHH) (CAT#: HPAB-0357-YC-VHH)
Provided are anti-BMP4 antibodies binding to a conformational epitope located within the BMPRla binding site of BMP4 and pharmaceutical uses thereof.
Specific Inquiry
SPR
Figure 5 Kinetic analysis for BMP binding.
Binding of VHHs to the different BMPs was tested in a direct SPR assay. The graphs show two replicate ligand injections at just one concentration of ligand.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
SPR
Figure 6 Kinetic analysis for BMP binding.
Epitope binning of VHH-coated chip plates. BMP4 (1.0 mg/mL) was injected on the chip followed by the injection of a second VHH (2.0 mg/mL) or the corresponding controls.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
Inhib
Figure 7 VHH biheads inhibit endogenous BMP function.
BMP activity of conditioned media of organoids cultured at the indicated conditions for 3 days.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
Inhib
Figure 8 VHH biheads inhibit endogenous BMP function.
Number of organoids formed in cultures of mouse intestinal organoids grown for 5 days at the indicated conditions.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
Inhib
Figure 9 Anti-BMP4 VHHs inhibit cancer-derived BMP signals.
C2C12 cells were incubated with conditioned media of the indicated cancer cell lines. C4C4, C8C8, or human Fc-Noggin were added at the same time at a concentration of 500 ng/mL for 16 hours.
Calpe, S., Wagner, K., El Khattabi, M., Rutten, L., Zimberlin, C., Dolk, E., ... & Krishnadath, K. K. (2015). Effective inhibition of bone morphogenetic protein function by highly specific llama-derived antibodies. Molecular cancer therapeutics, 14(11), 2527-2540.
Specifications
- Host Species
- Llama
- Derivation
- The VHH library
- Type
- Llama VHH
- Specificity
- Human BMP4
- Species Reactivity
- Human
- Clone
- HPAB-0357-YC-VHH
- Applications
- Surface plasmon resonance, Inhibition
- Related Disease
- Colorectal cancer
Applications
- Application Notes
- The antibody was validated for Surface plasmon resonance, Inhibition. For details, refer to Published Data.
Target
- Alternative Names
- Bone Morphogenetic Protein 4; Bone Morphogenetic Protein 2B; BMP-2B; BMP2B; BMP-4; BMP2B1; MCOPS6; OFC11; Zyme; DVR4
- Gene ID
- 652
- UniProt ID
- P12644
Related Resources
TGF-β Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| ZG-0339F | Mouse Anti-BMP4 Recombinant Antibody (ZG-0339F) | WB, ELISA | Mouse IgG |
| VS3-FY162 | Recombinant Rabbit Anti-BMP4 Antibody (clone R01-2G4) | WB, ICC, IF, IP | Rabbit IgG |
| VS4-WK50 | Rabbit Anti-BMP4 Recombinant Antibody (clone 19B7) | ELISA, WB, IF | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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