Recombinant Anti-human BMX Intrabody [Tat] (CAT#: IAB-B077(T))
Recombinant anti-BMX intrabody in fusion with HIV Tat works within cells to bind intracellur antigen BMX, expressed in 293T cells. These antibodiess bind specifically to recombinant BMX and efficiently block its kinase activity. When expressed in transformed cells, these antibodiess associated tightly with Etk, leading to significant blockade of Etk enzymatic activity and inhibition of clonogenic cell growth in soft agar.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HUVEC TERT2
(Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1048/1751_G2_18_cr58009842c82e5_selected.jpg
(Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1048/1751_G2_18_cr58009842c82e5_selected.jpg
Figure 2 Epididymis
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1048/2306_A_1_6.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1048/2306_A_1_6.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1048/2306_B_8_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1048/2306_B_8_5.jpg
Figure 4 Colon
(Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1048/2306_A_9_3.jpg
(Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1048/2306_A_9_3.jpg
Figure 5 Testis
(Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1048/2306_A_6_6.jpg
(Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1048/2306_A_6_6.jpg
Figure 6 RNA cell line category: Cell line enriched (HUVEC TERT2)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000102010-BMX
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000102010-BMX
Specifications
- Immunogen
- human BMX non-receptor tyrosine kinase
- Host Species
- human
- Type
- scFv-Tat
- Cell Penetrating Peptide
- TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
- Species Reactivity
- human
- Applications
- Formats of immunological tests: Flow Cytometry; Immunofluorescence; Functional studies
Product Property
- Purity
- 95% by HPLC
- Storage
- ≤1 year at -20°C.
Target
- Alternative Names
- BMX; BMX non-receptor tyrosine kinase; ETK; PSCTK2; PSCTK3; cytoplasmic tyrosine-protein kinase BMX; NTK38 tyrosine kinase; BTK-like on X chromosome; Etk/Bmx cytosolic tyrosine kinase; epithelial and endothelial tyrosine kinase; bone marrow tyrosine kinas
- Gene ID
- 660
- UniProt ID
- P51813
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Intrabody Products
IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B077(A) | Recombinant Anti-human BMX Intrabody [(D-Arg)9] | CO-IP, FuncS | scFv-(D-Arg)9 |
| IAB-B077(G) | Recombinant Anti-human BMX Intrabody [+36 GFP] | IF, ELISA, FuncS | scFv-Tat |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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