Recombinant Mouse Anti-BNP Antibody (106.3) (CAT#: PABX-017)
Anti-BNP antibody is a Mouse antibody of IgG1 class that binds to an BNP. This antibody binds an epitope spanning residues 5-13 of the mature bioactive peptide.
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FuncS
Figure 1 Equilibrium binding titration of Alexa488-BNP 5-13 with mAb 106.3 at various temperatures.
The dissociation constants are: 0.05 nM, 0.07 nM, 0.2 nM, 0.6 nM, 1.2 nM, 3.4 nM, 7.2 nM.
Longenecker, K. L., Ruan, Q., Fry, E. H., Saldana, S. C., Brophy, S. E., Richardson, P. L., & Tetin, S. Y. (2009). Crystal structure and thermodynamic analysis of diagnostic mAb 106.3 complexed with BNP 5‐13 (C10A). Proteins: Structure, Function, and Bioinformatics, 76(3), 536-547.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native Human BNP
- Species Reactivity
- Human
- Clone
- 106. 3
- Applications
- FuncS
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- This antibody has been reported for use in Functional Assay. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. should be carefully titrated in specific assays.
Target
- Alternative Names
- NPPB; natriuretic peptide B; BNP; B-type natriuretic peptid; anti-BNP
- Gene ID
- 4879
- UniProt ID
- P16860
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Mouse Antibodies
Rabbit Monoclonal Antibodies
IgG Antibodies
ScFv Antibodies
Fab Antibodies
ScFv-Fc Chimeras
IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-017-S (P) | Recombinant Mouse Anti-BNP Antibody scFv Fragment (106.3) | WB, ELISA, IF, FuncS | scFv |
| PABX-017-F (E) | Recombinant Mouse Anti-BNP Antibody Fab Fragment (106.3) | WB, ELISA, IF, FuncS | Fab |
Customer Reviews and Q&As
Customer Review
Q&As
Great for ELISA Assay
Using the recombinant mouse anti-BNP antibody (106.3), we have detected the high concentration BNP peptide. This excited result provided valuable guidance and support for our work in the future.
Suitable for IF Staining
Benefited by this antibody, our immunofluorescence staining experiment obtained ideal results. Target protein showed accurate localization to vesicles in the cell line that used in our experiment. It displayed strong fluorescence signal in a relatively short exposure time that can be accurately identified under the confocal microscope.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABX-017, RRID: AB_3111692)
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For Research Use Only. Not For Clinical Use.
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