Recombinant Mouse Anti-BfAChE Antibody (Fab410) (CAT#: PABL-375)
Recombinant Mouse Antibody (Fab410) is capable of binding to BfAChE , expressed in Chinese Hamster Ovary cells (CHO).This antibody could inhibit EeAChE and BfAChE by binding to their peripheral sites.
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Figure 1 SDS-PAGE analysis of AChE antibody Fab410.
Electrophoresis patterns obtained by SDS-PAGE in non-reducing conditions (12.5% PhastGel, left) and native-PAGE (7.5% PhastGel, center) with migration from the cathode (top) toward the anode (bottom), and by isoelectric focusing (pI 3-9 PhastGel, right). The three Fabs are more homogenous in mass than in charge, a feature that likely result from variations in the C-terminus generated by papaine cleavage of the CH chain; yet, all isoforms bind EeAChE equally, as verified by a native-PAGE mobility shift assay (not shown). The neutral average pI of Fab408 and cationic average pIs of Fab403 and Fab410 are evident.
Bourne, Y., Renault, L., Essono, S., Mondielli, G., Lamourette, P., Boquet, D., ... & Marchot, P. (2013). Molecular characterization of monoclonal antibodies that inhibit acetylcholinesterase by targeting the peripheral site and backdoor region. PLoS One, 8(10), e77226.
Specifications
- Immunogen
- Brachylophus fasciatus acetylcholinesterase
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native B. fasciatus AChE
- Species Reactivity
- B. fasciatus
- Clone
- Fab410
- Applications
- WB 1:1000-1:10000
ELISA 1:1000-1:20000
FuncS Assay-depended
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- Assay-depended
Target
- Alternative Names
- AChE; acetylcholinesterase
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-31-S(P) | Recombinant Anti-human ACHE Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| PSBL-004 | Mouse Anti-ACHE Recombinant Antibody (clone Ab408); scFv Fragment | WB, ELISA, FuncS | Mouse scFv |
| PSBL-024 | Recombinant Mouse Anti-BfAChE Antibody scFv Fragment (mAb410) | WB, ELISA, FuncS | scFv |
| PSBL-374 | Mouse Anti-ACHE Recombinant Antibody (clone Fab408); scFv Fragment | WB, ELISA, FuncS | Mouse scFv |
| PSBL-375 | Recombinant Mouse Anti-BfAChE Antibody scFv Fragment (Fab410) | WB, ELISA, FuncS | scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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