Recombinant Anti-Human CALCRL VHH Single Domain Antibody
CAT#: NAB-338-VHH
Anti-Human CALCRL VHH Single Domain Antibody is a recombinant protein produced in E. coli.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46515/1057_C8_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human skin
Immunohistochemical staining of human skin shows strong cytoplasmic positivity in epidermal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8070/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line AF22
Immunofluorescent staining of human cell line AF22 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/46515/1595_A10_2_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8070/21164_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8070/21164_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8070/21164_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8070/21164_A_6_6.jpg
Normal Tissue
Figure 8 Skin
Fibroblasts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Keratinocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Langerhans
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Melanocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8070/21164_B_8_1.jpg
❮
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Specifications
- Immunogen
- Human Calcitonin gene-related peptide Receptor
- Host Species
- Llama
- Type
- Llama VHH
- Species Reactivity
- Human
- Molecule Class
- VHH Single Domain Antibody
- Applications
- This product is suitable for use in IHC, IP, FC, Neut, FUNC and most other immunological methods.
Product Property
- Storage
- At -20°C for one year.
Target
- Alternative Names
- CALCRL; calcitonin receptor-like; calcitonin gene-related peptide type 1 receptor; CGRPR; CRLR; CGRP type 1 receptor; calcitonin receptor-like receptor;
REVIEWS AND Q&AS
CITATIONS
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Customer Review
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Excellent for Functional Assays
This antibody was instrumental in our functional studies involving CALCRL. The purity was impressive, and it worked effectively in neutralization experiments as well. The product arrived in good condition, stored at -20°C as recommended, and maintained activity throughout our project timeline.
Versatile Llama VHH Antibody
We utilized this recombinant Llama VHH antibody for our immunohistochemistry assays targeting the Calcitonin gene-related peptide receptor. The single domain format provided excellent penetration and signal clarity in our tissue samples. It is highly stable and performed well in our protocols.
Q&As
-
What is the host species and production method for this antibody?
A: This is a Recombinant Anti-Human CALCRL VHH Single Domain Antibody. It is raised in Llama and produced in E. coli. It is a single domain antibody, which offers unique properties for binding and stability.
-
What applications is this antibody suitable for?
A: Our product NAB-338-VHH is suitable for use in Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC), Neutralization (Neut), and Functional Assays (FUNC), as well as most other immunological methods.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# NAB-338-VHH, RRID: AB_3714099)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "CALCRL"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3237z | Mouse Anti-CALCRL Recombinant Antibody (MOB-3237z) | IHC, ICC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3237z-F(E) | Recombinant Anti-Human CALCRL Antibody Fab Fragment | IF, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3237z-S(P) | Recombinant Anti-Human CALCRL Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-091CL | Human Anti-CALCRL Recombinant Antibody (TAB-091CL) | PK, IP, FC | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-087CL | Afuco™ Anti-CALCRL Recombinant Antibody (AFC-087CL), ADCC Enhanced | ELISA, WB | Human IgG1 |
| CAT | Product Name | Application | Type |
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| MOB-038CQ | Rabbit Anti-Human CALCRL Polyclonal Antibody | WB, IHC-P, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP010-YC | Human Anti-CALCRL Recombinant Antibody (HPAB-AP010-YC) | FC, Block, Neut | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP011-YC | Human Anti-CALCRL Recombinant Antibody (HPAB-AP011-YC) | FC, Block, Neut | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP012-YC | Human Anti-CALCRL Recombinant Antibody (HPAB-AP012-YC) | FC, Block, Neut | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP010-YC-S(P) | Human Anti-CALCRL Recombinant Antibody; scFv Fragment (HPAB-AP010-YC-S(P)) | FC, Block, Neut | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP011-YC-S(P) | Human Anti-CALCRL Recombinant Antibody; scFv Fragment (HPAB-AP011-YC-S(P)) | FC, Block, Neut | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP012-YC-S(P) | Human Anti-CALCRL Recombinant Antibody; scFv Fragment (HPAB-AP012-YC-S(P)) | FC, Block, Neut | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP013-YC-S(P) | Human Anti-CALCRL Recombinant Antibody; scFv Fragment (HPAB-AP013-YC-S(P)) | FC, Block, Neut | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP010-YC-F(E) | Human Anti-CALCRL Recombinant Antibody; Fab Fragment (HPAB-AP010-YC-F(E)) | FC, Block, Neut | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP011-YC-F(E) | Human Anti-CALCRL Recombinant Antibody; Fab Fragment (HPAB-AP011-YC-F(E)) | FC, Block, Neut | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP012-YC-F(E) | Human Anti-CALCRL Recombinant Antibody; Fab Fragment (HPAB-AP012-YC-F(E)) | FC, Block, Neut | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP013-YC-F(E) | Human Anti-CALCRL Recombinant Antibody; Fab Fragment (HPAB-AP013-YC-F(E)) | FC, Block, Neut | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY38 | AbPlus™ Anti-CALCRL Magnetic Beads (VS-0424-XY38) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC285 | Recombinant Anti-CALCRL Vesicular Antibody, EV Displayed (VS-0425-YC285) | ELISA, FC, Cell-uptake |
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