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    Recombinant Anti-human CASP3 Intrabody [+36 GFP] (CAT#: IAB-B002(G))

    Recombinant anti-CASP3 intrabody in fusion with +36GFP works within cells and specifically binds to pro-apoptotic protein CASP3, expressed in CHO cells. These intrabodies are useful drugs on their own as well as a means for identifying small compound drugs for degenerative diseases involving oxidative-stress-induced apoptosis.

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    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

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    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2643/2013_E2_2_selected.jpg
    Figure 2 IHC staining of human adrenal gland
    Figure 2 IHC staining of human adrenal gland
    (Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in granular pattern in cortical cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/91/ihc_selected.jpg
    Figure 3 IF staining of human cell line A-431
    Figure 3 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & mitochondria.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2643/2_C1_1_red_green.jpg
    Figure 4 IF staining of human cell line U-251 MG
    Figure 4 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & mitochondria.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2643/3_C1_1_red_green.jpg
    Normal Tissue
    Figure 5 Colon
    Figure 5 Colon
    (Glandular cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8381/20562_A_9_3.jpg
    Figure 6 Testis
    Figure 6 Testis
    (Leydig cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Preleptotene spermatocytes
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8381/20562_A_6_6.jpg
    Figure 7 Lymph node
    Figure 7 Lymph node
    (Germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Non-germinal center cells
    Staining: Medium
    Intensity: Strong
    Quantity: <25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8381/20562_A_8_8.jpg
    RNA Expression
    Figure 8 RNA cell line category: Cell line enhanced (Karpas-707)
    Figure 8 RNA cell line category: Cell line enhanced (Karpas-707)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164305-CASP3
    Figure 8 RNA cell line category: Cell line enhanced (Karpas-707)
    Figure 8 RNA cell line category: Cell line enhanced (Karpas-707)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164305-CASP3

    Specifications

    • Immunogen
    • human caspase 3, apoptosis-related cysteine peptidase
    • Host Species
    • llama
    • Type
    • vhh-(+36GFP)
    • Cell Penetrating Peptide
    • +36 GFP
      GFP with a theoretical net charge of +36 (+36 GFP) penetrated several different mammalian cell lines at very low (nanomolar) concentrations. Cationic peptides and proteins have been observed to penetrate cells, and both natural and synthetic cell-penetrating peptides are widely used as delivery vehicles. However, whereas a covalent link between the cell-penetrating peptide and its cargo is typically required.
    • Species Reactivity
    • human
    • Applications
    • Antibody assay: Immunocytochemistry; Neutralization; Functional studies

    Product Property

    • Purity
    • >95% as determined by analysis by SDS-PAGE
    • Storage
    • Store at -20°C. Avoid multiple freeze/thaw cycles.

    Target

    • Alternative Names
    • CASP3; caspase 3, apoptosis-related cysteine peptidase; CPP32; SCA-1; CPP32B; caspase-3; CASP-3; CPP-32; apopain; procaspase3; protein Yama; PARP cleavage protease; cysteine protease CPP32; SREBP cleavage activity 1; caspase 3, apoptosis-related cysteine

    Related Resources

    • Related Signaling Pathways
    • Related Diseases

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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    Single Domain Antibody Products Intrabody Products Rabbit Monoclonal Antibody Products IgG Antibody Products Antibody Magnetic Beads Non-Human Primate (NHP) Antibody Products Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
    CAT Product Name Application Type
    NABG-037 Recombinant Anti-Human CASP3 VHH Single Domain Antibody WB, ELISA, IHC, FuncS Llama VHH

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