Recombinant Anti-human CASP3 Intrabody [Tat]
CAT#: IAB-B002(T)
Recombinant anti-CASP3 intrabody in fusion with HIV Tat works within cells and specifically binds to pro-apoptotic protein CASP3, expressed in CHO cells. These intrabodies are useful drugs on their own as well as a means for identifying small compound drugs for degenerative diseases involving oxidative-stress-induced apoptosis.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2643/2013_E2_2_selected.jpg
Subcellular Location
Figure 2 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in granular pattern in cortical cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/91/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2643/2_C1_1_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2643/3_C1_1_red_green.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8381/20562_A_9_3.jpg
Normal Tissue
Figure 6 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8381/20562_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8381/20562_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (Karpas-707)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164305-CASP3
❮
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Specifications
- Immunogen
- human caspase 3, apoptosis-related cysteine peptidase
- Host Species
- llama
- Type
- vhh-Tat
- Cell Penetrating Peptide
- TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
- Species Reactivity
- human
- Applications
- Used for immunoassay techniques such as: Enzyme-linked Immunosorbent Assay; Western blot; Immunocytochemistry; Functional studies
Product Property
- Purity
- >95% as judged by SDS-polyacrylamide gel electrophoresis
- Storage
- ≤6 months at 4°C; ≥6 months at -20°C.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Lot Number:
See other products for "CASP3"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-037 | Recombinant Anti-Human CASP3 VHH Single Domain Antibody | WB, ELISA, IHC, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B002(A) | Recombinant Anti-human CASP3 Intrabody [(D-Arg)9] | WB, ICC, FuncS | vhh-(D-Arg)9 |
| IAB-B002(G) | Recombinant Anti-human CASP3 Intrabody [+36 GFP] | ICC, Neut, FuncS | vhh-(+36GFP) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0475 | Hi-Affi™ Rabbit Anti-CASP3 Recombinant Antibody (clone DS475AB) | IF, IHC-Fr, IHC-P, WB | Rabbit IgG |
| MOR-4541 | Hi-Affi™ Rabbit Anti-CASP3 Recombinant Antibody (clone TH50DS) | WB, IF, ICC, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0580J | Rabbit Anti-CASP3 Recombinant Antibody (clone 5B2) | ELISA, WB, IHC, IF | Rabbit IgG |
| VS3-FY191 | Recombinant Rabbit Anti-CASP3 Antibody (clone R05-2D6) | WB, ICC, IF | Rabbit IgG |
| VS3-FY192 | Recombinant Rabbit Anti-CASP3 Antibody (clone R02-7C2) | WB, IHC-F, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS3-FY193 | Recombinant Rabbit Anti-CASP3 Antibody (clone R04-5C8) | WB | Rabbit IgG |
| VS3-FY194 | Recombinant Rabbit Anti-CASP3 Antibody (clone R08-6F2) | WB, IHC-P, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY41 | AbPlus™ Anti-CASP3 Magnetic Beads (CBACN-011) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY63 | Mouse Anti-NHP CASP3 Recombinant Antibody (VS-1024-XY63) | WB, IHC, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC127 | CytoStream™ Rabbit Anti-CASP3 Recombinant Antibody (VS13-YC127) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1001 | Anti-CASP3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1002 | Anti-Mouse CASP3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1003 | Anti-Porcine CASP3 Immunohistochemistry Kit | IHC |
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