Recombinant Anti-human CASP3 Intrabody [(D-Arg)9]
CAT#: IAB-B002(A)
Recombinant anti-CASP3 intrabody linked to (D-Arg)9 works within cells and specifically binds to pro-apoptotic protein CASP3, expressed in CHO cells. These intrabodies are useful drugs on their own as well as a means for identifying small compound drugs for degenerative diseases involving oxidative-stress-induced apoptosis.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2643/2013_E2_2_selected.jpg
Subcellular Location
Figure 2 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in granular pattern in cortical cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/91/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2643/2_C1_1_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2643/3_C1_1_red_green.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8381/20562_A_9_3.jpg
Normal Tissue
Figure 6 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8381/20562_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8381/20562_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (Karpas-707)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164305-CASP3
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Specifications
- Immunogen
- human caspase 3, apoptosis-related cysteine peptidase
- Host Species
- llama
- Type
- vhh-(D-Arg)9
- Cell Penetrating Peptide
- (D-Arg)9
It has been proven that polyarginine peptides composed of nine arginine amino acids (Arg-9) are able to translocate across cells very efficiently. This raises the question if molecular dynamics simulations would show a similar pathway for this highly hydrophilic and charged peptide as predicted for the Tat peptide. The simplicity of Arg-9 over other CPPs also helps isolate the role of the Arg sidechain on the translocation without the effect of other sidechains. Therefore, in this work we study computationally the translocation of Arg-9 through a lipid bilayer and show that the translocation mechanism is the same as for the Tat peptide.
- Species Reactivity
- human
- Applications
- Antibody assay: Western blot; Immunocytochemistry; Functional studies
Product Property
- Purity
- >98% by SDS-PAGE and HPLC analysis
- Storage
- 4°C or -20°C, avoid repeated freezing and thawing.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Lot Number:
See other products for "CASP3"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-037 | Recombinant Anti-Human CASP3 VHH Single Domain Antibody | WB, ELISA, IHC, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B002(G) | Recombinant Anti-human CASP3 Intrabody [+36 GFP] | ICC, Neut, FuncS | vhh-(+36GFP) |
| IAB-B002(T) | Recombinant Anti-human CASP3 Intrabody [Tat] | ELISA, WB, ICC, FuncS | vhh-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0421MZ | Mouse Anti-CASP3 Recombinant Antibody (clone 42B2078) | IHC-P, WB | Mouse IgG1 |
| MRO-0241-CN | Rabbit Anti-CASP3 Recombinant Antibody (clone CBACN-090) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-0580J | Rabbit Anti-CASP3 Recombinant Antibody (clone 5B2) | ELISA, WB, IHC, IF | Rabbit IgG |
| VS3-XY163 | Mouse Anti-CASP3 Recombinant Antibody (clone 4H2B10B2) | ELISA, WB, FC | Mouse IgG1 |
| VS3-XY164 | Mouse Anti-CASP3 Recombinant Antibody (clone 7E5G1) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0475 | Hi-Affi™ Rabbit Anti-CASP3 Recombinant Antibody (clone DS475AB) | IF, IHC-Fr, IHC-P, WB | Rabbit IgG |
| MOR-4541 | Hi-Affi™ Rabbit Anti-CASP3 Recombinant Antibody (clone TH50DS) | WB, IF, ICC, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY41 | AbPlus™ Anti-CASP3 Magnetic Beads (CBACN-011) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY63 | Mouse Anti-NHP CASP3 Recombinant Antibody (VS-1024-XY63) | WB, IHC, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC127 | CytoStream™ Rabbit Anti-CASP3 Recombinant Antibody (VS13-YC127) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1001 | Anti-CASP3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1002 | Anti-Mouse CASP3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1003 | Anti-Porcine CASP3 Immunohistochemistry Kit | IHC |
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