Hi-Affi™ Rabbit Anti-CAST Recombinant Antibody (clone DS483AB) (CAT#: MOR-0483)
Recombinant Rabbit anti-CAST monoclonal antibody for IHC-P, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to plasma membrane, cytosol & endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36882/857_F12_1_selected.jpg
(Immunofluorescent staining of human cell line A549 shows localization to plasma membrane, cytosol & endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36882/857_F12_1_selected.jpg
Figure 2 IF staining of human cell line HaCaT
(Immunofluorescent staining of human cell line HaCaT shows localization to cytosol & endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75906/1908_B9_2_selected.jpg
(Immunofluorescent staining of human cell line HaCaT shows localization to cytosol & endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75906/1908_B9_2_selected.jpg
Figure 3 Esophagus
(High expression in esophagus)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_B_5_1_val_selected.jpg
(High expression in esophagus)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_B_5_1_val_selected.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_A_8_5.jpg
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_A_8_5.jpg
Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_A_4_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_A_4_6.jpg
Figure 6 Skeletal muscle
(Myocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_B_2_6.jpg
(Myocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_B_2_6.jpg
Figure 7 Lymph node
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_A_8_8.jpg
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36881/136703_A_8_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000153113-CAST
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000153113-CAST
Specifications
- Immunogen
- A synthetic peptide derived from near the C-terminus of Human Calpastatin protein.
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- Rabbit IgG
- Species Reactivity
- Human
- Clone
- DS483AB
- Applications
- IHC-P
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Calpastatin; Sperm BS-17 Component; Calpain Inhibitor; BS-17; PLACK
- Gene ID
- 831
- UniProt ID
- P20810
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2319z | Mouse Anti-CAST Recombinant Antibody (clone 4B3) | WB, FC, IHC | Mouse IgG1 |
| MOB-1964MZ | Mouse Anti-CAST Recombinant Antibody (clone OTI1E7) | ELISA, WB | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
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