Afuco™ Anti-Human CCL20 ADCC Recombinant Antibody (GSK3050002), ADCC Enhanced (CAT#: AFC-092CL)

Figure 1 Linear PK distribution of GSK3050002 in serum and blister fluid.

(A) GSK3050002 in serum, (B) GSK3050002 in blister fluid. Data is presented as mean + SD (serum: n = 12 for placebo, n = 6 for GSK3050002; blister fluid: n = 9–11 for placebo, n = 3–6 for GSK3050002 with the exception of 0.1 mg kg−1; n = 1–3).

Bouma, G.,Zamuner,S.,Hicks, K.,Want,A.,Oliveira, J.,Choudhury, A.,& Fernando, D.(2017).CCL20 neutralization by a monoclonal antibody in healthy subjects selectively inhibits recruitment of CCR6+ cells in an experimental suction blister.British Journal of Clinical Pharmacology,83(9),1976-1990.

Figure 2 Dose dependent increase over time of complexed and free CCL20 in serum and blister.

(A) GSK3050002/CCL20 complex in serum, (B) GSK3050002/CCL20 complex in blister fluid, (C) free CCL20 by ELISA in serum and (D) free CCL20 by ELISA in blister fluid. (E) Free CCL20 comparison of ELISA, MSD and protein A/G depletion of samples of a single subject that received 20 mg kg−1 GSK3050002. (F) Comparison of predicted vs. actual GSK3050002/CCL20 concentration data. Data is presented as mean + SEM in A–D (serum: n = 12 for placebo, n = 6 for GSK3050002; blister fluid: n = 9–11 for placebo, n = 3–6 for GSK3050002). Data from D of 0.1 mg kg−1 cohort is not included as this was not measured with the validated assay. Data in F shows actual data as data points with error bars representing SEM and the lines represent predicted data with 95% confidence intervals as shaded areas.

Bouma, G.,Zamuner,S.,Hicks, K.,Want,A.,Oliveira, J.,Choudhury, A.,& Fernando, D.(2017).CCL20 neutralization by a monoclonal antibody in healthy subjects selectively inhibits recruitment of CCR6+ cells in an experimental suction blister.British Journal of Clinical Pharmacology,83(9),1976-1990.

Figure 3 GSK3050002 mediates selective inhibition of CCR6+ cells in a suction blister model.

(A) Representative histograms showing dose-dependent inhibition of CCR6+ T-cells. (B) CCR6+ T-cells, (C) CD3+CCR6+

Bouma, G.,Zamuner,S.,Hicks, K.,Want,A.,Oliveira, J.,Choudhury, A.,& Fernando, D.(2017).CCL20 neutralization by a monoclonal antibody in healthy subjects selectively inhibits recruitment of CCR6+ cells in an experimental suction blister.British Journal of Clinical Pharmacology,83(9),1976-1990.