Hi-Affi™ Rabbit Anti-CDC25C Recombinant Antibody (clone DS601AB)
CAT#: MOR-0601
Recombinant Rabbit anti-CDC25C monoclonal antibody for ICC, IP, WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66991/1250_B5_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3800/if_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3800/10841_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3800/10841_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3800/10841_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3800/10841_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000158402-CDC25C
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Specifications
- Immunogen
- A synthetic phospho-peptide corresponding to residues surrounding Ser216 of human Cdc25C.
- Host Species
- Rabbit
- Derivation
- Phage display library
- Type
- Rabbit IgG
- Specificity
- Human CDC25C
- Species Reactivity
- Human
- Clone
- DS601AB
- Applications
- ICC, IP, WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Cell Division Cycle 25C; EC 3.1.3.48; Cell Division Cycle 25 Homolog C (S. Cerevisiae); Protein Phosphatase 1, Regulatory Subunit 60; Cell Division Cycle 25 Homolog C (S. Pombe); Dual Specificity Phosphatase CDC25C; Dual Specificity Phosphatase Cdc25C; Phosphotyrosine Phosphatase; Protein Phosphatase 1; Regulatory Subunit 60; Mitosis Inducer CDC25; PPP1R60; CDC25
- Gene ID
- 995
- UniProt ID
- P30307
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "CDC25C"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2469MZ | Recombinant Mouse Anti-Human CDC25C Antibody | ELISA, IHC-P, WB | Mouse antibody |
| ZG-0400F | Mouse Anti-CDC25C Recombinant Antibody (ZG-0400F) | WB, IHC, ELISA | Mouse IgG |
| ZG-0603J | Rabbit Anti-CDC25C Recombinant Antibody (clone 3E6) | ELISA, WB, IHC, IF, IP | Rabbit IgG |
| VS3-CJ867 | Mouse Anti-CDC25C Recombinant Antibody (VS3-CJ867) | WB, IHC | Mouse IgG2a, κ |
| VS3-XY3302 | Rabbit Anti-CDC25C Recombinant Antibody (clone R02-6D2) | WB, IHC-P, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0110MZ | Chicken Anti-CDC25c Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1259 | Anti-CDC25C Immunohistochemistry Kit | IHC |
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