Mouse Anti-CDC25C Recombinant Antibody (ZG-0400F)
CAT#: ZG-0400F
This product is a mouse antibody that recognizes CDC25C.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-CDC25C Recombinant Antibody (ZG-0400F) in WB.
Western blot analysis was performed using CDC25C monoclonal antibodies against HeLa (1), K562 (2), PC-3 (3), HEK293 (4) and Raw264.7 (5) cell lysates.
IHC
Figure 2 Mouse Anti-CDC25C Recombinant Antibody (ZG-0400F) in IHC.
The CDC25C monoclonal antibody was used for immunohistochemical analysis of paraffin-embedded human testis with AEC staining.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66991/1250_B5_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3800/if_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3800/10841_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3800/10841_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3800/10841_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3800/10841_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000158402-CDC25C
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Specifications
- Immunogen
- Recombinant fragment of human CDC25C expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CDC25C
- Species Reactivity
- Human
- Applications
- Western Blot, Immunohistochemistry, Enzyme-linked Immunosorbent Assay
- Conjugate
- NONE
Product Property
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/200-1/1000
ELISA: 1/10000
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "CDC25C"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2469MZ | Recombinant Mouse Anti-Human CDC25C Antibody | ELISA, IHC-P, WB | Mouse antibody |
| MRO-1764-CN | Rabbit Anti-CDC25C Polyclonal Antibody (MRO-1764-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-XY3302 | Rabbit Anti-CDC25C Recombinant Antibody (clone R02-6D2) | WB, IHC-P, ICC, IF, IP, FC | Rabbit IgG |
| VS7-HM660 | Mouse Anti-CDC25C Recombinant Antibody (clone 1F12) | WB, IHC | Mouse IgG1 |
| VS7-HM661 | Mouse Anti-CDC25C Recombinant Antibody (clone CBL131HM) | WB, IHC-p, IF, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0110MZ | Chicken Anti-CDC25c Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0601 | Hi-Affi™ Rabbit Anti-CDC25C Recombinant Antibody (clone DS601AB) | ICC, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1259 | Anti-CDC25C Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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