Rabbit Anti-CDC25C Recombinant Antibody (clone 3E6) (CAT#: ZG-0603J)
This product is a rabbit antibody that recognizes CDC25C.
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WB
Figure 1 Rabbit Anti-CDC25C Antibody (ZG-0603J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate
All lanes: Cdc25C antibody at 1.65μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 54, 52, 49, 46 KDa
Observed band size: 60 KDa
IHC
Figure 2 Rabbit Anti-CDC25C Antibody (ZG-0603J) in IHC
IHC image of ZG-0603J diluted at 1:165 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 3 Rabbit Anti-CDC25C Antibody (ZG-0603J) in IF
Immunofluorescence staining of Hela cells with ZG-0603J at 1:55, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
IP
Figure 4 Rabbit Anti-CDC25C Antibody (ZG-0603J) in IP
Immunoprecipitating CDC25C in HEK293 whole cell lysate
Lane 1: Rabbit control IgG instead of ZG-0603J in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0603J (3μg) + HEK293 whole cell lysate (500μg)
Lane 3: HEK293 whole cell lysate (20μg)
Specifications
- Immunogen
- A synthesized peptide derived from human CDC25C
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CDC25C
- Species Reactivity
- Human
- Clone
- 3E6
- Applications
- ELISA, WB, IHC, IF, IP
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, IP:1:200-1:1000
Target
- Alternative Names
- CDC25; PPP1R60; M-phase inducer phosphatase 3; CDC25 homolog C; dual specificity phosphatase CDC25C; mitosis inducer CDC25; phosphotyrosine phosphatase; protein phosphatase 1, regulatory subunit 60
- Gene ID
- 995
- UniProt ID
- P30307
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "CDC25C"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2469MZ | Recombinant Mouse Anti-Human CDC25C Antibody | ELISA, IHC-P, WB | Mouse antibody |
| ZG-0400F | Mouse Anti-CDC25C Recombinant Antibody (ZG-0400F) | WB, IHC, ELISA | Mouse IgG |
| VS3-XY3302 | Rabbit Anti-CDC25C Recombinant Antibody (clone R02-6D2) | WB, IHC-P, ICC, IF, IP, FC | Rabbit IgG |
| VS7-HM660 | Mouse Anti-CDC25C Recombinant Antibody (clone 1F12) | WB, IHC | Mouse IgG1 |
| VS7-HM661 | Mouse Anti-CDC25C Recombinant Antibody (clone CBL131HM) | WB, IHC-p, IF, ELISA | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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