Rabbit Anti-CDC25C Recombinant Antibody (clone 3E6) (CAT#: ZG-0603J)

Figure 1 Rabbit Anti-CDC25C Antibody (ZG-0603J) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate
All lanes: Cdc25C antibody at 1.65μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 54, 52, 49, 46 KDa
Observed band size: 60 KDa

Figure 2 Rabbit Anti-CDC25C Antibody (ZG-0603J) in IHC

IHC image of ZG-0603J diluted at 1:165 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Figure 3 Rabbit Anti-CDC25C Antibody (ZG-0603J) in IF

Immunofluorescence staining of Hela cells with ZG-0603J at 1:55, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).

Figure 4 Rabbit Anti-CDC25C Antibody (ZG-0603J) in IP

Immunoprecipitating CDC25C in HEK293 whole cell lysate
Lane 1: Rabbit control IgG instead of ZG-0603J in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0603J (3μg) + HEK293 whole cell lysate (500μg)
Lane 3: HEK293 whole cell lysate (20μg)