Mouse Anti-CEACAM6 Recombinant Antibody (HPAB-0288-CN) (CAT#: HPAB-0288-CN)
This product is a recombinant mouse antibody that recognizes CEACAM6. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
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ELISA
Figure 1 Mouse Anti-CEACAM6 Antibody (HPAB-0288-CN) in ELISA
ELISA analysis of HPAB-0288-CN was performed by coating with human CEACAM6 protein (His tag). Then blocked with BSA and incubated with HPAB-0288-CN. The HRP-conjugated goat anti-IgG was used as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 2 Mouse Anti-CEACAM6 Antibody (HPAB-0288-CN) in WB
Western blot analysis of HPAB-0288-CN was performed by loading human CEACAM6 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0288-CN and a HRP goat anti-IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.
DB
Figure 3 Mouse Anti-CEACAM6 Antibody (HPAB-0288-CN) in DB
Dot Blot analysis of HPAB-0288-CN was performed by coating with human CEACAM6 protein (His tag).
HPAB-0288-CN incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CEACAM6
- Species Reactivity
- Human
- Applications
- WB, IHC
- Related Disease
- Cancer, particularly cancer of epithelial or myeloid origin
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (Non-Specific Cross Reacting Antigen); Normal Cross-Reacting Antigen; NCA; Non-Specific Crossreacting Antigen; Cluster Of Differentiation 66c; CD66c Antigen; CD66c; CEAL
- Gene ID
- 4680
- UniProt ID
- P40199
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-042 | Recombinant Anti-Human CEACAM6 VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
| TAB-004LC-VHH | Anti-Human CEACAM6 Recombinant Antibody (TAB-004LC-VHH) | ELISA, WB, FC, Inhib, FuncS, ICC, SPR | Single domain antibody |
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For Research Use Only. Not For Clinical Use.
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