Mouse Anti-CEACAM6 Recombinant Antibody (HPAB-0288-CN) (CAT#: HPAB-0288-CN)

Figure 1 Mouse Anti-CEACAM6 Antibody (HPAB-0288-CN) in ELISA

ELISA analysis of HPAB-0288-CN was performed by coating with human CEACAM6 protein (His tag). Then blocked with BSA and incubated with HPAB-0288-CN. The HRP-conjugated goat anti-IgG was used as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Mouse Anti-CEACAM6 Antibody (HPAB-0288-CN) in WB

Western blot analysis of HPAB-0288-CN was performed by loading human CEACAM6 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0288-CN and a HRP goat anti-IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.

Figure 3 Mouse Anti-CEACAM6 Antibody (HPAB-0288-CN) in DB

Dot Blot analysis of HPAB-0288-CN was performed by coating with human CEACAM6 protein (His tag).
HPAB-0288-CN incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control