Human Anti-CSF2 Recombinant Antibody (clone MB007) (CAT#: PABL-481)

Recombinant Human Antibody (MB007) is capable of binding to GM-CSF, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-GM-CSF mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-GM-CSF proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This is a high-affinity anti-(human GM-CSF) autoantibody isolated from a patient suffering from PAP which shows only modest neutralization of GM-CSF bioactivity.

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FuncS

Figure 1 Influence of ionic strength on binding of MB007 to GM-CSF.

hGM-CSF was fluorescently labelled with Alexa Fluor® 647, and binding to MB007 Fab was analysed by MST. Binding isotherms show high salt-dependence of complex formation. Results are the mean fluorescence differences calculated from three independent titration series acquired at 298 K by locally applying a temperature gradient ofapproximately 2 K to each individual measurement. Curves were fitted using a non-linear single-site equation. Binding curves are coloured blue (10 mM NaCl), green (100 mM NaCl), grey (500 mM NaCl), orange (750 mM NaCl) and red (1 M NaCl). a.u., arbitrary units.

Blech, M., Seeliger, D., Kistler, B., Bauer, M. M., Hafner, M., Hörer, S., ... & Park, J. E. (2012). Molecular structure of human GM-CSF in complex with a disease-associated anti-human GM-CSF autoantibody and its potential biological implications. Biochemical Journal, 447(2), 205-215.


Specifications

  • Immunogen
  • Human colony stimulating factor 2 (granulocyte-macrophage)
  • Host Species
  • Human
  • Type
  • Human IgG
  • Specificity
  • Human CSF2
  • Species Reactivity
  • Human
  • Clone
  • MB007
  • Applications
  • Neut

Applications

  • Application Notes
  • This antibody has been reported for use in Function Assay.

Target

  • Alternative Names
  • CSF2; colony stimulating factor 2 (granulocyte-macrophage); GMCSF; granulocyte-macrophage colony-stimulating factor; CSF; molgramostin; sargramostim; granulocyte macrophage-colony stimulating factor

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "CSF2"

See other products for "Clone MB007"


For Research Use Only. Not For Clinical Use.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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