Human Anti-CSF2 Recombinant Antibody (clone MB007) (CAT#: PABL-481)

Recombinant Human Antibody (MB007) is capable of binding to GM-CSF, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-GM-CSF mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-GM-CSF proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This is a high-affinity anti-(human GM-CSF) autoantibody isolated from a patient suffering from PAP which shows only modest neutralization of GM-CSF bioactivity.


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  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
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FuncS

Figure 1 Influence of ionic strength on binding of MB007 to GM-CSF.

Figure 1 Influence of ionic strength on binding of MB007 to GM-CSF.

hGM-CSF was fluorescently labelled with Alexa Fluor® 647, and binding to MB007 Fab was analysed by MST. Binding isotherms show high salt-dependence of complex formation. Results are the mean fluorescence differences calculated from three independent titration series acquired at 298 K by locally applying a temperature gradient ofapproximately 2 K to each individual measurement. Curves were fitted using a non-linear single-site equation. Binding curves are coloured blue (10 mM NaCl), green (100 mM NaCl), grey (500 mM NaCl), orange (750 mM NaCl) and red (1 M NaCl). a.u., arbitrary units.

Blech, M., Seeliger, D., Kistler, B., Bauer, M. M., Hafner, M., Hörer, S., ... & Park, J. E. (2012). Molecular structure of human GM-CSF in complex with a disease-associated anti-human GM-CSF autoantibody and its potential biological implications. Biochemical Journal, 447(2), 205-215.


Specifications

  • Immunogen
  • Human colony stimulating factor 2 (granulocyte-macrophage)
  • Host Species
  • Human
  • Type
  • Human IgG
  • Specificity
  • Human CSF2
  • Species Reactivity
  • Human
  • Clone
  • MB007
  • Applications
  • Neut

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been reported for use in Function Assay.

Target

  • Alternative Names
  • CSF2; colony stimulating factor 2 (granulocyte-macrophage); GMCSF; granulocyte-macrophage colony-stimulating factor; CSF; molgramostin; sargramostim; granulocyte macrophage-colony stimulating factor

Related Resources

  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "Clone MB007"

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Chimeric Antibody

CAT Product Name Application Type
TAB-184CT Human Anti-CSF2 Recombinant Antibody (TAB-184CT) ELISA, FC, WB, Block Chimeric (Mouse/Human) IgG1, κ
TAB-184CT-S(P) Human Anti-CSF2 Recombinant Antibody; scFv Fragment (TAB-184CT-S(P)) ELISA, FC, WB, Block Human scFv
TAB-184CT-F(E) Human Anti-CSF2 Recombinant Antibody; Fab Fragment (TAB-184CT-F(E)) ELISA, FC, WB, Block Chimeric (Mouse/Human) Fab

Blocking Antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-4580 Hi-Affi™ Rabbit Anti-CSF2 Recombinant Antibody (clone TH92DS) WB Rabbit IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-259 Afuco™ Anti-CSF2 ADCC Recombinant Antibody (Namilumab), ADCC Enhanced IP, IF, FuncS, FC, Neut, ELISA ADCC enhanced antibody
AFC-TAB-H44 Afuco™ Anti-CSF2 ADCC Recombinant Antibody (Lenzilumab), ADCC Enhanced Neut, ELISA, IF, IP, FuncS, FC ADCC enhanced antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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