Human Anti-CSF2 Recombinant Antibody (clone MB007)

Figure 1 Influence of ionic strength on binding of MB007 to GM-CSF.

hGM-CSF was fluorescently labelled with Alexa Fluor® 647, and binding to MB007 Fab was analysed by MST. Binding isotherms show high salt-dependence of complex formation. Results are the mean fluorescence differences calculated from three independent titration series acquired at 298 K by locally applying a temperature gradient ofapproximately 2 K to each individual measurement. Curves were fitted using a non-linear single-site equation. Binding curves are coloured blue (10 mM NaCl), green (100 mM NaCl), grey (500 mM NaCl), orange (750 mM NaCl) and red (1 M NaCl). a.u., arbitrary units.

Blech, M., Seeliger, D., Kistler, B., Bauer, M. M., Hafner, M., Hörer, S., ... & Park, J. E. (2012). Molecular structure of human GM-CSF in complex with a disease-associated anti-human GM-CSF autoantibody and its potential biological implications. Biochemical Journal, 447(2), 205-215.