Recombinant Human Anti-DENV E proteins Antibody (CAT#: PABL-063)

Figure 1 Direct ELISA comparing the binding profile of E1 mG2a and E1 mG2a/hG3 for 14c10 hG1.

The wells were coated with 14c10 hG1 and E1 mG2a and E1 mG2a/hG3 were added. Results represent average of two independent experiments performed in duplicates.

Lim, S.Y., Lisowska, M.M., Hanson, B.J., et al. (2015). The Molecular Engineering of an Anti-Idiotypic Antibody for Pharmacokinetic Analysis of a Fully Human Anti-Infective. PLOS ONE.

Figure 2 Determination of the binding specificity of E1 mG2a/hG3 for 14c10 hG1.

The antigens (14c10 hG1, 3H5hG1, D29 hG1 and commercially available human IgG1) were coated and the detector E1 mG2a/hG3 was used.

Lim, S.Y., Lisowska, M.M., Hanson, B.J., et al. (2015). The Molecular Engineering of an Anti-Idiotypic Antibody for Pharmacokinetic Analysis of a Fully Human Anti-Infective. PLOS ONE.

Figure 3 Inhibition of binding of 14c10 hG1 to DENV-1 with E1 Fab.

The wells were coated at a constant concentration with 4G2 mG2a as a capture for DENV-1. The antibodies (14c10 hG1 and D29 hG1) were used at a constant concentration and were preincubated with E1 Fab at room temperature, before the addition to DENV-1. Results are an average of two independent experiments performed in duplicates.

Lim, S.Y., Lisowska, M.M., Hanson, B.J., et al. (2015). The Molecular Engineering of an Anti-Idiotypic Antibody for Pharmacokinetic Analysis of a Fully Human Anti-Infective. PLOS ONE.

Figure 4 Direct ELISA comparing the binding profile of E1 mG2a and E1 mG2a/hG3 for 14c10 hG1.

AG129 mice were infected with DENV-1 WP74 (genotype 4). Ten microgram of 14c10 hG1 was inoculated intraperitoneally (i.p.) per mouse as therapy two days after infection (A- C) and as prophylaxis two days before infection (D- F). Blood was collected at various time points and the viremia level (B, E) and the concentration of serum 14c10 hG1 (C, F) was determined with plaque assay and sandwich ELISA respectively. Each group (14c10 hG1 treated and PBS control) consisted of five AG129 mice.

Lim, S.Y., Lisowska, M.M., Hanson, B.J., et al. (2015). The Molecular Engineering of an Anti-Idiotypic Antibody for Pharmacokinetic Analysis of a Fully Human Anti-Infective. PLOS ONE.