Recombinant Human Anti-DENV E proteins Antibody (2D22) (CAT#: PABL-065)

Figure 1 DENV2 serotype-specific hMAbs use multiple quaternary epitopes.

DENV2 hMAbs 2D22, 1L12 and 3F9 were assessed for their ability to bind whole DENV2 virions (A), DENV2 rE (B), and DENV2 rEDIII (C). Positive control (+) hMAb is DVC10.16, a DENV2 hMAb that uses a simple A-strand epitope contained entirely within EDIII and 5J7 is a DENV3 serotype-specific hMAb as a negative control (-). (D) Blockade of binding assay where hMAbs 2D22, 1L12 or 3F9 were assessed for their ability to block 2D22-AP from binding to DENV2.

Gallichotte, E.N., Baric, T.J., Widman, D.G., et al. (2018). Human dengue virus serotype 2 neutralizing antibodies target two distinct quaternary epitopes. PLOS ONE.

Figure 2 2D22 was assessed for their ability to bind recombinant DENVs in ELISA binding assays. Dotted line in ELISA represents the background signal.

Gallichotte, E.N., Baric, T.J., Widman, D.G., et al. (2018). Human dengue virus serotype 2 neutralizing antibodies target two distinct quaternary epitopes. PLOS ONE.

Figure 3 2D22 was assessed for their ability to neutralize recombinant DENVs in Vero-81 Focus Reduction Neutralization Tests (FRNT).

FRNT50 represents the concentration of antibody required to neutralize 50% of infection. # = virus was not neutralized at highest concentration of hMAb tested (5 ng/μl).

Gallichotte, E.N., Baric, T.J., Widman, D.G., et al. (2018). Human dengue virus serotype 2 neutralizing antibodies target two distinct quaternary epitopes. PLOS ONE.

Figure 4 ELISA of sE-cvD from DENV serotypes 2, 3 and 4 and ZIKV with the indicated mAbs (left panel). Control antigens, 4DIII, 4DI/DII and 4sE wt were also reacted with the same set of antibodies (right panel).

Slon Campos, J.L., Marchese, S., Rana, J., Mossenta, M., Poggianella, M., Bestagno, M., Burrone, O.R. (2017). Temperature-dependent folding allows stable dimerization of secretory and virus-associated E proteins of Dengue and Zika viruses in mammalian cells. Scientific Report, 7(966).

Figure 5. Cytofluorimetric analysis of HEK-293T cells transfected with the membrane display sE-cvD versions of all four DENV serotypes and ZIKV at 37 °C or 28 °C and reacted with the indicated mAbs. Mock-transfected HEK-293T cells incubated with each mAb served as controls.

Slon Campos, J.L., Marchese, S., Rana, J., Mossenta, M., Poggianella, M., Bestagno, M., Burrone, O.R. (2017). Temperature-dependent folding allows stable dimerization of secretory and virus-associated E proteins of Dengue and Zika viruses in mammalian cells. Scientific Report, 7(966).

Figure 1 Recombinant Human Anti-DENV E proteins Antibody (PABL-065) in SDS-PAGE

SDS-PAGE analysis of PABL-065 in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions.

Figure 2 Recombinant Human Anti-DENV E proteins Antibody (PABL-065) in HPLC

The purity of PABL-065 in HPLC is>95%.