Recombinant Human Anti-DENV E proteins Antibody (5J7)
CAT#: PABL-067
Recombinant Human Antibody (5J7) is capable of binding to DENV E proteins, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-DENV E proteins mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-DENV E proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody is exceptionally potent, neutralizing 50% of virus at nanogram-range antibody concentration.
Published Data
Tested Data
Figure 1 Serotype-specific strongly neutralizing E protein binding human MAbs.
(A) The ability of purified human MAb 5J7 to neutralize the four serotypes of DENV is shown over a concentration range. (B) The ability of purified human MAb 2D22 to neutralize the four serotypes of dengue virus is shown over a concentration range. (C) Purified human MAb 5J7 neutralization of serotype 3 virus over a detailed, broader range of halving dilutions. (D) Purified human MAb 2D22 neutralization of serotype 2 virus over a detailed range of halving dilutions. Flow cytometric neutralization assays were performed using U937-DC-SIGN cells.
Smith, S.A., Zhou, Y., Olivarez, N.P., et al. Persistence of Circulating Memory B Cell Clones with Potential for Dengue Virus Disease Enhancement for Decades following Infection. Journal of Virology, 2665-2675.
Figure 2 ELISA of sE-cvD from DENV serotypes 2, 3 and 4 and ZIKV with the indicated mAbs (left panel). Control antigens, 4DIII, 4DI/DII and 4sE wt were also reacted with the same set of antibodies (right panel).
Slon Campos, J.L., Marchese, S., Rana, J., Mossenta, M., Poggianella, M., Bestagno, M., Burrone, O.R. (2017). Temperature-dependent folding allows stable dimerization of secretory and virus-associated E proteins of Dengue and Zika viruses in mammalian cells. Scientific Report, 7(966).
Figure 3 Cytofluorimetric analysis of HEK-293T cells transfected with the membrane display sE-cvD versions of all four DENV serotypes and ZIKV at 37 °C or 28 °C and reacted with the indicated mAbs. Mock-transfected HEK-293T cells incubated with each mAb served as controls.
Slon Campos, J.L., Marchese, S., Rana, J., Mossenta, M., Poggianella, M., Bestagno, M., Burrone, O.R. (2017). Temperature-dependent folding allows stable dimerization of secretory and virus-associated E proteins of Dengue and Zika viruses in mammalian cells. Scientific Report, 7(966).
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SDS-PAGE
Figure 1 Recombinant Human Anti-DENV E proteins Antibody (PABL-067) in SDS-PAGE
SDS-PAGE analysis of PABL-067 in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions.
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Specifications
- Immunogen
- Dengue virusEnvelope proteins
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native DENV E proteins
- Species Reactivity
- DENV
- Clone
- 5J7
- Applications
- ELISA, Neut, FuncS
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The antibody was validated for Neut and ELISA. For details, refer to published data.
Target
- Alternative Names
- E proteins; Envelope proteins; Env; dengue virus; DENV
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Affordable and Quality
This antibody is affordable without compromising on quality. It delivers excellent results equivalent to more expensive options, making it a cost-effective choice.
Increased Precision and Intricacy
Strong and precise binding was made possible by the affinity-purified nature, which raised the reliability of the outcomes in a range of applications.
Adaptable with Varying Detergents
It functioned consistently even in the presence of mild detergents employed in investigations on membrane proteins.
Q&As
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What are the benefits of using this antibody in a multiplex assay?
A: Multiplex assays allow simultaneous detection of multiple targets, saving time and sample material, and providing comprehensive data in a single experimental setup.
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Can I use this antibody with both fresh and formalin-fixed samples?
A: Yes, but each sample type may require different preparation protocols, such as antigen retrieval for formalin-fixed samples, to optimize binding and detection.
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How do I determine the antibody's concentration in a stock solution?
A: Use UV spectroscopy at 280 nm or a Bradford protein assay, following standard protocols to determine the concentration of the antibody in your stock solution.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABL-067, RRID: AB_3111624)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 5J7"
- CAT
- Product Name
See other products for "E proteins"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-063 | Recombinant Human Anti-DENV E proteins Antibody | WB, Neut, FuncS | IgG |
| PABL-064 | Recombinant Human Anti-DENV E proteins Antibody (1F4) | Neut, FuncS | IgG |
| PABL-065 | Recombinant Human Anti-DENV E proteins Antibody (PABL-065) | WB, BL, FuncS | IgG |
| PABL-066 | Recombinant Chimpanzee Anti-DENV E proteins Antibody (5H2) | WB, ELISA, FuncS | IgG |
| PABC-224 | Recombinant Mouse Anti-DENV-2 EDIII Antibody (E104) | Neut | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-063 | Recombinant Human Anti-DENV E proteins Antibody scFv Fragment | WB, Neut, FuncS | scFv |
| PSBL-064 | Recombinant Human Anti-DENV E proteins Antibody scFv Fragment (1F4) | Neut, FuncS | scFv |
| PSBL-069 | Human Anti-E proteins Recombinant Antibody; scFv Fragment (PSBL-069) | WB, ELISA, Neut, FuncS | Human scFv |
| PSBL-072 | Recombinant Mouse Anti-DENV-1 Envelope protein DIII Antibody scFv Fragment (E106) | WB, ELISA, FuncS | scFv |
| PFBC-224 | Recombinant Mouse Anti-DENV-2 EDIII Antibody Fab Fragment (E104) | Neut | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-063 | Recombinant Human Anti-DENV E proteins Antibody Fab Fragment | WB, Neut, FuncS | Fab |
| PFBL-064 | Recombinant Human Anti-DENV E proteins Antibody Fab Fragment (1F4) | Neut, FuncS | Fab |
| PFBL-065 | Recombinant Human Anti-DENV E proteins Antibody Fab Fragment (PFBL-065) | WB, BL, FuncS | Fab |
| PFBL-066 | Recombinant Chimpanzee Anti-DENV E proteins Antibody Fab Fragment (5H2) | WB, ELISA, FuncS | Fab |
| PFBL-067 | Recombinant Human Anti-DENV E proteins Antibody Fab Fragment (5J7) | ELISA, Neut, FuncS | Fab |
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