Recombinant Human Anti-DENV E proteins Antibody (EDE2 A11) (CAT#: PABL-069)

Recombinant Human Antibody (EDE2 A11) is capable of binding to DENV E proteins, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-DENV E proteins mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-DENV E proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.

  • Published Data
  • Datasheet
  • MSDS
  • COA

Figure 1 Neutralization curves using three antibodies each from the three subsets FLE, EDE1 and EDE2.

Results represent the mean ± s.e.m. of four independent experiments performed in triplicate for PF13 and in duplicate for HD78788 strains. The two ZIKV strains are in bright colours, blue and red, respectively. The neutralization data for the four DENV serotypes are given here for comparison. Note that the DENV-4 strain used was a natural isolate lacking the N153 glycosylation site (N−).

Barba-Spaeth, G., Dejnirattisai, W., Rouvinski, A., Vaney, M.C., Medits, I., Sharma, A. (2016). Structural basis of potent Zika–dengue virus antibody cross-neutralization. Nature, 536, 48-53.

Figure 2 ZIKV sE reactivity with human recombinant full-length antibodies FLE P6B10, EDE1 C8 and EDE2 A11. Left, binding properties were monitored by biolayer interferometry (BLI) on Octet-Red (ForteBio).

The normalized response values expressed as fraction of binding site occupancy are plotted against concentrations of ZIKV sE dimer shown at logarithmic scale. Lines denote global curve fits used for Kd evaluation.

Barba-Spaeth, G., Dejnirattisai, W., Rouvinski, A., Vaney, M.C., Medits, I., Sharma, A. (2016). Structural basis of potent Zika–dengue virus antibody cross-neutralization. Nature, 536, 48-53.


Specifications

  • Immunogen
  • Dengue virusEnvelope proteins
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG
  • Specificity
  • Tested positive against native DENV E proteins
  • Species Reactivity
  • DENV
  • Clone
  • EDE2 A11
  • Applications
  • WB, ELISA, Neut, FuncS

Applications

  • Application Notes
  • The antibody was validated for Neut. For details, refer to published data.

Target

  • Alternative Names
  • E proteins; Envelope proteins; Env; dengue virus; DENV

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "E proteins"

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

Send Inquiry

  • Verification code
    Click image to refresh the verification code.
© 2007 - 2019 Creative BioLabs All Rights Reserved
  • 0
  • 0

Cart