Human Anti-DPP4 Recombinant Antibody (HPAB-0215-YC) (CAT#: HPAB-0215-YC)
This product is a recombinant humanized antibody that recognizes DPP4. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography. The anti-proliferative effect of specified recombinant human MAb was analyzed in Jurkat CD26+ cells and the most inhibitory concentration was at 5 μg/mL. The antibody has an a discontinuous or conformational epitope. It had high reactivity against peptides: YSLRWISDHEYLY, YVKQWRHSYTASY, TWSPVGHKLAYVW, EEEVFSAYSALWW and RISLQWLRRIQNY.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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WB
Figure 1 Anti-DPP4 Recombinant Antibody (HPAB-0215-YC) in WB
Western blot analysis of HPAB-0215-YC (2 μg/mL) was performed with Recombinant Human DPP4/CD26 Protein (His Tag). The HRP-Anti-Human IgG (H+L) (1: 6000) was used as a secondary antibody.
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg
DB
Figure 2 Anti-DPP4 Recombinant Antibody (HPAB-0215-YC) in DB
Dot Blot analysis of HPAB-0215-YC (2 μg/mL) was performed by coating with Recombinant Human DPP4/CD26 Protein (His Tag). The HRP-Anti-Human IgG (H+L) (1: 6000) was used as a secondary antibody.
ELISA
Figure 3 Anti-DPP4 Recombinant Antibody (HPAB-0215-YC) in ELISA
ELISA analysis of HPAB-0215-YC was performed by coating with Recombinant Human DPP4/CD26 Protein (His Tag). Then blocked with BSA, and incubated with HPAB-0215-YC. The HRP-conjugated goat Anti-Human IgG was used as a secondary antibody (1:5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG1
- Specificity
- Human DPP4
- Species Reactivity
- Human
- Applications
- ELISA, Inhib, FuncS
- Related Disease
- Diseases associated with expression of CD26
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Dipeptidyl-Peptidase 4; Adenosine Deaminase Complexing Protein 2; Dipeptidylpeptidase IV (CD26, Adenosine Deaminase Complexing Protein 2); T-Cell Activation Antigen CD26; Dipeptidyl Peptidase IV; EC 3.4.14.5; DPP IV; ADCP-2; ADCP2; ADABP; TP103; CD26; Dipeptidylpeptidase 4; CD26 Antigen; DPPIV
- Gene ID
- 1803
- UniProt ID
- P27487
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1041 | Hi-Affi™ Rabbit Anti-DPP4 Recombinant Antibody (clone DS1041AB) | WB | Rabbit IgG |
Customer Reviews and Q&As
Customer Review
Q&As
Background That Is Not Reactive
The antibody appears to have produced an excellent non-reactive background. It guarantees that the information gathered is precise and unique to the target protein.
Simple to Follow Procedures
The antibody comes with user-friendly, easy-to-follow methods that guarantee successful experimental outcomes without requiring a lot of debugging.
Reliable for Prolonged Research
Long-term investigations have shown that this antibody is quite dependable, performing consistently throughout time. It's a stable and reliable product.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-0215-YC, RRID: AB_3111372)
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For Research Use Only. Not For Clinical Use.
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