Recombinant Human Antibody (mAb23) is capable of binding to ECD, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-ECD mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-ECD proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody blocks glucagon binding and inhibits basal receptor activity, indicating that it is an inverse agonist and that the ECD can negatively regulate receptor activity independent of ligand binding.
Figure 1 Antibody mAb1 blocks glucagon-induced PEPCK gene expression in human hepatocytes.
Average IC50s (nM) from two experiments are 0.15, 1.5, and 0.4 for mAb1, mAb7, and mAb23, respectively.
Koth, C. M., Murray, J. M., Mukund, S., Madjidi, A., Minn, A., Clarke, H. J., ... & Rondon, J. (2012). Molecular basis for negative regulation of the glucagon receptor. Proceedings of the National Academy of Sciences, 109(36), 14393-14398.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
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CAT | Product Name | Application | Type |
---|---|---|---|
PABL-459 | Human Anti-ECD Recombinant Antibody (clone mAb1) | ELISA, WB, FuncS | Human IgG |
MOB-1843MZ | Recombinant Mouse Anti-Human ECD Antibody | ELISA, WB | Mouse antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-456 | Human Anti-ECD Recombinant Antibody (clone mAb1); Fab Fragment | ELISA, WB, FuncS | Human Fab |
PFBL-457 | Human Anti-ECD Recombinant Antibody (clone mAb23); Fab Fragment | ELISA, WB, FuncS | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBL-456 | Human Anti-ECD Recombinant Antibody (clone mAb1); scFv Fragment | ELISA, WB, FuncS | Human scFv |
PSBL-457 | Human Anti-ECD Recombinant Antibody (clone mAb23); scFv Fragment | ELISA, WB, FuncS | Human scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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