Human Anti-ECD Recombinant Antibody (clone mAb23)
CAT#: PABL-460
Recombinant Human Antibody (mAb23) is capable of binding to ECD, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-ECD mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-ECD proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody blocks glucagon binding and inhibits basal receptor activity, indicating that it is an inverse agonist and that the ECD can negatively regulate receptor activity independent of ligand binding.
Published Data
Gene Expression
Figure 1 Antibody mAb1 blocks glucagon-induced PEPCK gene expression in human hepatocytes.
Average IC50s (nM) from two experiments are 0.15, 1.5, and 0.4 for mAb1, mAb7, and mAb23, respectively.
Koth, C. M., Murray, J. M., Mukund, S., Madjidi, A., Minn, A., Clarke, H. J., ... & Rondon, J. (2012). Molecular basis for negative regulation of the glucagon receptor. Proceedings of the National Academy of Sciences, 109(36), 14393-14398.
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6465/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6465/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6465/17913_A_7_3.jpg
Normal Tissue
Figure 4 Testis
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6465/17913_A_6_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6465/17913_A_9_8.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000122882-ECD
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Specifications
- Immunogen
- Human ecdysoneless homolog (Drosophila)
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human ECD
- Species Reactivity
- Human
- Clone
- mAb23
- Applications
- ELISA, WB, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone mAb23"
- CAT
- Product Name
See other products for "ECD"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-459 | Human Anti-ECD Recombinant Antibody (clone mAb1) | ELISA, WB, FuncS | Human IgG |
| MOB-1843MZ | Recombinant Mouse Anti-Human ECD Antibody | ELISA, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-456 | Human Anti-ECD Recombinant Antibody (clone mAb1); Fab Fragment | ELISA, WB, FuncS | Human Fab |
| PFBL-457 | Human Anti-ECD Recombinant Antibody (clone mAb23); Fab Fragment | ELISA, WB, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-456 | Human Anti-ECD Recombinant Antibody (clone mAb1); scFv Fragment | ELISA, WB, FuncS | Human scFv |
| PSBL-457 | Human Anti-ECD Recombinant Antibody (clone mAb23); scFv Fragment | ELISA, WB, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC363 | AbPlus™ Anti-ECD Magnetic Beads (VS-0724-YC363) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY721 | Anti-ECD Immunohistochemistry Kit | IHC |
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